通讯机构：
[Wang, PY; Liu, XL ] C;Chinese Acad Sci, Key Lab Design & Assembly Funct Nanostruct, Fujian Inst Res Struct Matter, Fuzhou 350002, Peoples R China.;Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China.;Hosp Fujian Med Univ, United Innovat Mengchao Hepatobiliary Technol Key, Mengchao Hepatobiliary, Fuzhou 350025, Peoples R China.
摘要：
Triggering the healing process of drug-resistant bacteria-infected wounds has attracted great attention due to global morbidity that may induce gangrene, amputation, and even death. Here, a chitin derivative, carboxymethyl chitosan (CMC), tannic acid (TA), and Cu(2+) were used for hydrogel engineering. Using sodium bicarbonate as the neutralizer and reductant, hydrogen bonds between CMC and TA and in situ Cu(OH)(2) generation via ion coordination force between Cu(2+) and TA facilitated the synthesis of CMC/TA/Cu hydrogel. Cu(2+) and TA release, cytotoxicity, in vitro cell migration, angiogenesis, and antidrug-resistant bacteria were measured. Besides, wound closure was evaluated in vivo using the methicillin-resistant Staphylococcus aureus (MRSA)-infected excisional dermal wound mouse model. Negligible toxicity was observed both in vitro and in vivo. Dermal cell migration and angiogenesis were significantly enhanced. In vivo, the CMC/TA/Cu hydrogel induced effective re-epithelialization, collagen deposition, inflammatory alleviation, and MRSA inhibition during wound repair in mice. All these results confirmed that the CMC/TA/Cu hydrogel is a promising novel dressing for chronic wound healing in clinic.
摘要：
An A2B2O7‐type high‐entropy oxide is applied for a photoelectrochemical photodetector for the first time. The experimental results demonstrated the tunable photodetection performance of the as‐prepared photodetector. Interestingly, the photodetector shows excellent long‐term stability and there is only ≈5.00 % attenuation in performance even after the photodetector is placed after 6 months in the ambient environment. Abstract Optoelectronics with excellent long‐term stability is meaningful for practical applications. Herein, for the first time, an A2B2O7 type high‐entropy oxide of (La0.2Ce0.2Nd0.2Gd0.2Bi0.2)2Ti2O7 (ATO) is synthesized and applied for photoelectrochemical photodetection. The lattice distortion, highly dispersed metal composition, and exposed active sites of ATO are beneficial for the fast separation and transmission of photogenerated electron/hole pairs, endowing ATO‐based devices with good photodetection performance. Both the density functional theory calculations and the nondegenerate transient absorption spectroscopy demonstrate the good optoelectronic properties of ATO. The systematic experimental studies reveal the tunable photodetection capability of ATO‐based photodetector (PD) in the visible region. A photocurrent of 772.00 nA cm−2 and a responsivity of 4.02 µA W−1 can be achieved as the PD in 1.0 m KOH with the bias potential of 0.6 V. Importantly, the robust and reproducible ON/OFF signals of the PD can be verified and there is only ≈5.00% attenuation in photocurrent even after 6 months, revealing the great potential of high‐ entropy oxides for practical applications.
作者机构：
[Zeng, Qun] Department of Biochemistry and Molecular Biology, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China;[Jiang, Tingting] Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China. Electronic address: 554860529@qq.com
通讯机构：
[Jiang, Tingting] D;Department of Clinical Laboratory, The Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China. Electronic address:
期刊：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2024年146(7):4363-4368 ISSN：0002-7863
通讯作者：
Yang, Z;Huang, J
作者机构：
[Yang, Zhen; Yang, Z; Wang, Zheyuan; Song, Zhilin] Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Shenzhen 518055, Peoples R China.;[Yang, Zhen; Yang, Z; Wang, Zheyuan; Song, Zhilin] Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen 518055, Peoples R China.;[Huang, Jun] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Yang, Zhen; Yang, Z] Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci, Minist Educ, Beijing 100871, Peoples R China.;[Yang, Zhen; Yang, Z] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China.
通讯机构：
[Yang, Z ] P;[Huang, J ] U;Peking Univ, Shenzhen Grad Sch, State Key Lab Chem Oncogen, Shenzhen 518055, Peoples R China.;Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen 518055, Peoples R China.;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要：
The total syntheses of penicibilaenes A and B are described. The key step is the (t)BuOK/DMSO-mediated tandem 5-exo-dig Conia-ene type reaction and 6-exo-dig Conia-ene type reaction to install the tricyclic [6.3.1.0(1,5)] dodecane core of penicibilaenes from dibutynyl cyclohexanone in a single step, together with a sequence of copper-mediated conjugate addition and Crabtree's hydrogenation to forge the stereogenic centers at C5 and C2, respectively.
作者机构：
[Zhang, Yuexin; Mu, Xinxin; Zhang, Qinyi; Li, Chunquan; Song, Shuang; Zhang, Guorui; Song, Chao; Tang, Huifang; Yin, Mingxue; Zhang, Hang] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Yuexin; Mu, Xinxin; Zhang, Qinyi; Li, Chunquan; Song, Shuang; Zhang, Guorui; Song, Chao; Tang, Huifang; Yin, Mingxue; Zhang, Hang] Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Qinyi; Li, Chunquan; Song, Shuang; Zhang, Guorui; Yin, Mingxue] Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;[Zhang, Qinyi; Li, Chunquan; Song, Shuang; Zhang, Guorui; Yin, Mingxue] Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.;[Li, Chunquan] Univ South China, Hunan Prov Maternal & Child Hlth Care Hosp, Hengyang Med Sch, Key Lab Birth Defect Res & Prevent, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Li, CQ ; Tang, HF ; Tang, HF] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Multiom & Artificial Intelligen, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Sch Basic Med Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Hengyang Med Sch, MOE Key Lab Rare Pediat Dis, Hengyang 421001, Hunan, Peoples R China.
关键词：
gated blood-pool imaging;chromatin;genetics;mice;enhancer of transcription;candidate disease gene;single nucleotide polymorphism;crispr;genes;rna;genome;methylation
摘要：
Enhancer RNAs (eRNAs) transcribed from distal active enhancers serve as key regulators in gene transcriptional regulation. The accumulation of eRNAs from multiple sequencing assays has led to an urgent need to comprehensively collect and process these data to illustrate the regulatory landscape of eRNAs. To address this need, we developed the eRNAbase (http://bio.liclab.net/eRNAbase/index.php) to store the massive available resources of human and mouse eRNAs and provide comprehensive annotation and analyses for eRNAs. The current version of eRNAbase cataloged 10 399 928 eRNAs from 1012 samples, including 858 human samples and 154 mouse samples. These eRNAs were first identified and uniformly processed from 14 eRNA-related experiment types manually collected from GEO/SRA and ENCODE. Importantly, the eRNAbase provides detailed and abundant (epi)genetic annotations in eRNA regions, such as super enhancers, enhancers, common single nucleotide polymorphisms, expression quantitative trait loci, transcription factor binding sites, CRISPR/Cas9 target sites, DNase I hypersensitivity sites, chromatin accessibility regions, methylation sites, chromatin interactions regions, topologically associating domains and RNA spatial interactions. Furthermore, the eRNAbase provides users with three novel analyses including eRNA-mediated pathway regulatory analysis, eRNA-based variation interpretation analysis and eRNA-mediated TF-target gene analysis. Hence, eRNAbase is a powerful platform to query, browse and visualize regulatory cues associated with eRNAs. Graphical Abstract
作者：
Hu, Yu-wen;Sultana, Fozia;Balogun, M. -Sadeeq;Xiong, Tuzhi;Huang, Yongchao;...
期刊：
Nanoscale,2024年16(8):4325-4332 ISSN：2040-3364
通讯作者：
Xia, Y;Balogun, MS;Xiong, TZ
作者机构：
[Balogun, MS; Sultana, Fozia; Balogun, M. -Sadeeq; Hu, Yu-wen] Hunan Univ, Coll Mat Sci & Engn, Changsha 410082, Peoples R China.;[Xia, Yu] South China Univ Technol, Guangzhou Peoples Hosp 1, Sch Med, Dept Gastroenterol & Hepatol, Guangzhou 510180, Peoples R China.;[Xiong, TZ; Xiong, Tuzhi] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Huang, Yongchao] Guangzhou Univ, Inst Environm Res Greater Bay, Key Lab Water Qual & Conservat Pearl River Delta, Minist Educ, Guangzhou 510006, Peoples R China.
通讯机构：
[Xiong, TZ ] U;[Xia, Y ] S;[Balogun, MS ] H;South China Univ Technol, Guangzhou Peoples Hosp 1, Sch Med, Dept Gastroenterol & Hepatol, Guangzhou 510180, Peoples R China.;Hunan Univ, Coll Mat Sci & Engn, Changsha 410082, Peoples R China.
摘要：
Nickel nitride (Ni(3)N) is a promising electrocatalyst for the hydrogen evolution reaction (HER) owing to its excellent metallic features and has been demonstrated to exhibit considerable activity for water oxidation. However, its undesirable characteristics as an HER electrocatalyst due to its poor unfavourable d-band energy level significantly limit its water dissociation kinetics. Herein, the HER electrocatalytic activity of Ni(3)N was prominently enhanced via the simultaneous incorporation of bi-cations (vanadium (V) and iron (Fe), denoted as V-Fe-Ni(3)N). The optimized V-Fe-Ni(3)N displays impressive performance with an overpotential of 69 mV at 10 mA cm(-2) and good stability in 1.0 M KOH, which is remarkably better than pristine Ni(3)N, V-doped Ni(3)N, and Fe-doped Ni(3)N and considerably closer to a commercial Pt/C catalyst. Based on density functional theory (DFT) studies, V and Fe atoms not only serve as active sites for promoting water dissociation kinetics but also tune the electronic structure of Ni(3)N to achieve optimized hydrogen adsorption capabilities. This work presents an inclusive understanding of the rational designing of high-performance transition metal nitride-based electrocatalysts for hydrogen production. Its electrocatalytic performance can be significantly enhanced by doping transition metal cations.
摘要：
Constructing desirable morphology has been demonstrated to be an effective strategy for enhancing the capabilities of the covalent organic frameworks (COFs) for the processing of uranium. Traditional 3D-COFs only possess a limited number of structural nodes, not being the ideal structure for uranium adsorption, but 2D-COFs with rich structural nodes also suffer from reduced exposure of adsorption sites due to stacking during uranium treatment, which is not beneficial for achieving sustained and efficient adsorption. To this end, by polymerizing COFs on the silica carrier, followed by etching the silica carrier, and utilizing the conversion of nitrile to amidoxime groups as chelation sites, we designed and synthesized a hollow 3D-like structure of 2D-COFs (COF-TD-H-AO, similar to three-dimensional COFs) as adsorbents to capture the radionuclide uranium. Systematic studies were carried out for the adsorption characteristics of this material at different pH values and in the presence of interfering ions, as well as its adsorption thermodynamics and kinetics. Compared with COF-TD-AO (pure 2D-COFs) and SiO2@COF-TD-AO (unetched), COF-TD-H-AO exhibited a higher adsorption capacity as well as selective adsorption of uranium. This work provides an innovative perspective on material design and preparation by providing more adsorption sites in an effort to improve the capacity of adsorption and the selectivity of adsorption.
期刊：
PATHOLOGY RESEARCH AND PRACTICE,2024年253:155042 ISSN：0344-0338
通讯作者：
Lian, Gaojian;Su, ZH
作者机构：
[Li, Zheng; Lian, Gaojian; Su, Zehong; Gong, Huifang; Wu, Zhimin] Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Lian, GJ; Su, ZH ] U;Univ South China, Hengyang Med Sch, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
关键词：
Biomarker;Cancer therapy;Ferroptosis;NcRNAs;Programmed cell death
摘要：
Ferroptosis is a recently discovered cell programmed death. Extensive researches have indicated that ferroptosis plays an essential role in tumorigenesis, development, migration and chemotherapy drugs resistance, which makes it become a new target for tumor therapy. Non-coding RNAs (ncRNAs) are considered to control a wide range of cellular processes by modulating gene expression. Recent studies have indicated that ncRNAs regulate the process of ferroptosis via various pathway to affect the development of cancer. However, the regulation network remains ambiguous. In this review, we outlined the major metabolic processes of ferroptosis and concluded the relationship between ferroptosis-related ncRNAs and cancer progression. In addition, the prospect of ncRNAs being new therapeutic targets and early diagnosis biomarkers for cancer by regulating ferroptosis were presented, and the possible obstacles were also predicted. This could help in discovering novel cancer early diagnostic methods and therapeutic approaches.
摘要：
Four porous corundum-spinel aggregates were prepared from magnesia and alpha-Al2O3 micropowder via the Kirkendall effect. Effects of the particle size of magnesia (PSM, 0-74 mu m, 0-15 mu m, and 0-44 mu m) and firing temperature (1100-1600 degrees C) on the phase compositions, microstructures, pore characteristics of the prepared porous aggregates were investigated. As the firing temperature increased, the PSM influenced the reaction rate of magnesia and alumina, and the sintering process, leading to remarkable changes in the phase compositions, porosity, and microstructure with a negligible impact on the pore size distribution. As the PSM decreased from 0-74 mu m to 0-15 mu m, the apparent porosity initially increased and then decreased. Relatively compact cyclic spinels formed around the Kirkendall pores in aggregates with 0-74 mu m and 0-44 mu m magnesia added after fired at 1600 degrees C, as the expansion of spinel formation was inhibited by the high strength of the alumina matrix. Finally, the optimized product is an aggregate with 0-44 mu m magnesia added, which exhibits a high total porosity of 32.4%, a low bulk density of 2.69 g/cm3, and a thermal conductivity of 2.178 W/(m center dot K) at 1000 degrees C, as well as a high compressive strength of 302.5 MPa and a small median pore size of .46 mu m. This work provides a promising technique for preparing porous-corundum-based aggregates.
摘要：
Electro/photo/photoelectro-catalytic technologies have shown huge potential for U(VI) capture. However, current researches mainly focus on designing various electrode materials, but rarely on the significance of structure match and cooperative working mechanism of anode and cathode for U(VI) removal. Here, a multi-mechanism co-driven (MMCD) photoelectrochemical method, involving electrosorption, micro electric-field attraction, coordination and photoelectrocatalytic reduction, was originally proposed. Through purposeful design of an advanced asymmetrical photoelectrocatalytic system, consisting of 1D/2D TiO2/graphene (GTR) photoanode and xanthan gum-polyacrylic acid bound graphene (RGX) cathode, the MMCD system exhibited enhanced photoelectrocatalytic activity, fast electron transfer ability, and abundant binding active groups, which drove U (VI) ions to fast electro-migrate towards the surface of RGX cathode, then form micro electric-field attraction and coordination with electronegative O-containing groups, and finally be catalytically reduced into electroneutral sediments with the aid of photoelectrons from GTR anode. Ultimately, the MMCD system displayed a high removal rate of 90.5 % at 1.0 V, 1.7 times and 4.5 times that of electrochemical and photocatalytic methods. Its kinetic rate was also 71 % and 296 % faster than that of the latter two methods. Meanwhile, this system also exhibited an improved reduction efficiency of U(VI) to U(IV), high selectivity as well as good reusability.
摘要：
The signal pathway of TNF‐α inducing human umbilical vascular endothelial cells apoptosis. Abstract Endothelial cell apoptosis driven by inflammation (TNF‐α) plays a critical role in the pathogenesis of atherosclerosis, but the exact molecular mechanisms are not clearly elucidated. MicroRNA (miR)‐29 families (a/b/c) take important roles in pathophysiological processes of atherosclerosis, also the underlying mechanisms have not been fully clarified. The aims are to explore whether or not miR‐29 families mediate the apoptotic effects of TNF‐α on endothelial cells and uncover the underlying molecular mechanisms. In this study, MTT assay and flow cytometer analysis were employed respectively to determine the proliferation and apoptosis of human umbilical vascular endothelial cells (HUVECs) under TNF‐α exposure. Real‐time quantitative PCR and western blot were performed to detect the levels of target RNAs and proteins/their phosphorylation in HUVECs. TNF‐α could inhibit HUVEC proliferation and induce HUVEC apoptosis in a positive dose‐ and time‐dependent manner, with a similar way of miR‐29a upregulation, but no effects on miR‐29b/c. Upregulation of miR‐29a with its mimics enhanced the apoptotic effect of TNF‐α on HUVECs, but downregulation of miR‐29a using anti‐miR‐29a blocked up its apoptotic effect. MiR‐29a inhibited the expression of PI3Kp85α and Bcl‐2 and blocked up the signal transduction of PI3K/AKT/Bcl‐2 axis to mediate the apoptotic effect of TNF‐α on HUVECs. Mediating the inflammation‐driven endothelial cell apoptosis is an important biology mechanism by which miR‐29a promotes atherosclerosis and its complications. MiR‐29a will be a potential diagnostic and therapeutic target for atherosclerotic cardiovascular diseases; it is worthwhile to further study.
作者：
Tao Gong;Huanyi Liang;Xing Li;Guoqing Li;Fengyue Chen;...
期刊：
Separation and Purification Technology,2024年342:126963 ISSN：1383-5866
通讯作者：
Tao Gong<&wdkj&>HongQing Wang
作者机构：
[Tao Gong; Huanyi Liang; Xing Li; Guoqing Li; Fengyue Chen; HongQing Wang] College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
通讯机构：
[Tao Gong; HongQing Wang] C;College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
摘要：
Extracting uranium (U) from seawater is an important future energy development direction. Due to the shortcomings of traditional uranium adsorption systems, double-network hydrogel nanofibers were fabricated via electrospinning and steam crosslinking. These hydrogel nanofibers combined the unique beaded network structure of electrospun nanofibers and the flexibility of a double-network hydrogel. The stretched PAO molecular chains in the hydrogel nanofibers provided a larger specific surface area and more binding sites that could combine with U. According to the results, the hydrogel nanofibers exhibited high water permeability, high hydrophilicity, high adsorption efficiency, rapid filtration, and a high recycling rate during uranium extraction. Moreover, an average adsorption capacity of 134 mg-U/g was obtained after 30 h of immersion in dilute U-containing seawater via a flow-through column system. These new hydrogel nanofibers show great potential as a next-generation adsorbent for uranium extraction from seawater.
作者机构：
[Wu, Jia; Li, Fusheng; Fang, Likui; Duan, Chongwen; Xu, Xiangyun; Wang, Dan; Chen, Shan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Tang, Weiyang] Hangzhou Normal Univ, Sch Pharm, Hangzhou 311121, Peoples R China.
通讯机构：
[Li, FS ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要：
1,3-Enynes have been widely recognized as prevalent synthons for the construction of valuable allenes, 1,3-dienes, and propargylic derivatives. However, significant advancements have primarily focused on elucidating the ionic reaction mechanism. The selective difunctionalization of 1,3-enynes involving radicals remains an outstanding challenge in particular within the realm of radical 1,2-difunctionalization. Herein, we present a comprehensive account of the regio- and diastereoselective radical multicomponent 1,2-dialkylation of 1,3-enynes via photoexcited Hantzsch ester and chromium co-catalysis to furnish highly valuable homopropargylic alcohols. Our protocol shows excellent compatibility and 1,2-regioselectivity. Remarkably, this innovative approach obviates the need for external photocatalysts, stoichiometric metal reductants, or additional additives, offering a novel strategy for the valuable transformation of 1,3-enynes. We present a comprehensive account of the regio- and diastereoselective radical multicomponent 1,2-dialkylation of 1,3-enynes via photoexcited Hantzsch ester and chromium co-catalysis to furnish highly valuable homopropargylic alcohols.
摘要：
The use of biomass as a catalyst offers a cost-effective and efficient approach for various processes, including the activation of peroxymonosulfate (PMS) to degrade contaminants. In this study, we successfully obtained Fe and N co -doped graphene-like hierarchical porous structure carbon (Fe-N/BC) by using undaria pinnatifida (wakame) as the precursor. The Fe-N/BC catalyst was utilized to activate PMS and degrade tetracycline (TC). Characterization results demonstrated an even distribution of Fe and N on the surface of the porous structure carbon. Compared to BC@PMS and N/BC@PMS, the Fe-N/BC@PMS system exhibited excellent degradation performance, achieving an 83.9 % degradation of TC in just 5 min with an ultralow catalyst dosage (Ca = 0.05 g/L) and a high TC concentration (TC = 0.03 g/L). Furthermore, experiments on radicals quenching and electron paramagnetic resonance (EPR) analysis indicated that singlet oxygen (1O2), a non -radical species, was demonstrated as the primary reactive oxygen species (ROS). The degradation mechanical analysis suggested that zero-valent iron (Fe0), pyridinic N, graphitic N, and Fe-Nx in the Fe-N/BC were the reason for the high catalytic activity. Moreover, the degradation pathways of TC were suggested through the use of LC -MS. Additionally, the ECOSAR system was employed to assess the toxicity of the intermediate products. This study reveals the active site and its mechanism in Fe -N composite biocarbon catalysts, providing a new method for synthesizing algal biocarbon catalysts.
摘要：
The environmental hazards of microplastics have received widespread attention. However, the in-situ detection of microplastics, particularly in aquatic environments, has been challenged by the limitations of detection methods, the large-scale instruments, and small size. Herein, a photoelectrochemical sensor based on the protein corona-induced aggregation effect is designed for the detection of polystyrene microplastics. The sensor has advantages of high sensitivity, reproducibility, and detection capability. A linear detection range of 0.5-500μgmL(-1), a method detection limit of 0.06μgmL(-1), and a limit of quantification of 0.14μgmL(-1) are achieved. Furthermore, the relative standard deviations of intra-day and inter-day precision, ranging from 0.56% to 4.63% and 0.84%-3.36% are obtained. A digital multimeter was employed to construct a platform for the real-time detection in real water samples, streamlining the detection process and yielding clear results. We believe this sensor provides new insight for the in-situ real-time detection of microplastics and has broad applications for the analysis of microplastic pollution in aquatic environments.
摘要：
Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., k(cat)/K(m) = 720.42M(-1) s(-1)) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45±0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation (k(cat)/K(m) = 670.08M(-1) s(-1)), while H64D/V68I had the best degradation rate against ST (i.e., 99.45±0.23%). Our results demonstrate that SAs can be efficiently degraded by artificial peroxygenases constructed using a myoglobin scaffold. This therefore provides a simple and economical method for the biodegradation of SD and ST.
作者机构：
[Wang, Huamin; Lin, Ying-Wu; Chen, Ze-Yuan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China.;[Yuan, Hong; Tan, Xiangshi] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China.;[Gao, Shu-Qin; Sun, Li-Juan; Lin, Ying-Wu] Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China.;[Yu, Lu] Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China.
通讯机构：
[Lin, YW ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;Univ South China, Hengyang Med Sch, Hengyang 421001, Peoples R China.
关键词：
heme active site;heme proteins;protein engineering;covalent modification;X-ray structure
摘要：
Covalent modification: A facial approach was developed to regulate the heme active site of heme protein by cavity generation, followed by covalent modifications, as shown by the two case studies using myoglobin as a model protein. Abstract Using myoglobin (Mb) as a model protein, we herein developed a facial approach to modifying the heme active site. A cavity was first generated in the heme distal site by F46 C mutation, and the thiol group of Cys46 was then used for covalently linked to exogenous ligands, 1H‐1,2,4‐triazole‐3‐thiol and 1‐(4‐hydroxyphenyl)‐1H‐pyrrole‐2,5‐dione. The engineered proteins, termed F46C‐triazole Mb and F46C‐phenol Mb, respectively, were characterized by X‐ray crystallography, spectroscopic and stopped‐flow kinetic studies. The results showed that both the heme coordination state and the protein function such as H2O2 activation and peroxidase activity could be efficiently regulated, which suggests that this approach might be generally applied to the design of functional heme proteins.
摘要：
Multilayer MXene (Ti3C2) with large layer spacing, large specific surface area, and few surface end-groups-F was obtained by using tetrapropylammonium hydroxide intercalation. The morphology, structure, and physicochemical properties of the samples were analyzed by characterization means of XRD, FT-IR, SEM, and BET, and the removal ability of the samples for uranyl ions was investigated by the batch removal experiments. The experimental results show that the removal of U(VI) by the intercalated Ti3C2 is more in line with the quasi-secondary kinetic model and Langmuir isothermal model, which is heat-absorbing and spontaneous, and the maximum removal capacity of U(VI) reaches 399.18 mg/g. Finally, the characterization by means of FT-IR, XPS, etc. reveals that the removal of U(VI) is mainly due to the interaction of U(VI) with the laminar plate of the material, which is the main cause for the removal of U(VI) bound to the laminate hydroxyl groups of the material through surface complexation. The overall findings suggest that the intercalated Ti3C2 has potential applications in the efficient treatment of radioactive wastewater.
摘要：
Spent fuel shearing machines in nuclear power plants are important equipment for the head end of spent fuel reprocessing in power reactors. Condition monitoring and fault diagnosis play important roles in ensuring the safe operation of spent fuel shearing machines, avoiding serious accidents, and reducing their maintenance time and cost. Existing research on fault diagnosis of spent fuel shearing machines has some shortcomings: (a) the current research on fault diagnosis of shearing machines is small and diagnostic accuracy is not high. The research methodology of shearing machines needs to be updated; (b) the high difficulty in obtaining fault data and the often limited and highly informative fault data for shearing machines lead to low diagnostic performance. To solve these problems, this study constructs a residual network (ResNet) model based on Bayesian optimization (BO) and convolutional block attention module (CBAM). First, dual-channel difference method is introduced into the preprocessing of noise signals, and two data enhancements were applied to the Mel spectrograms used as inputs to the model. Second, the attention mechanism CBAM is introduced to improve the ResNet to enhance the deep feature extraction ability of the network, and the BO algorithm is used to train the hyperparameters, such as the optimizer, and retrain the network model after obtaining the optimal hyperparameters. Finally, the feasibility and effectiveness of the proposed model are verified through experiments on the noise signals of spent fuel shearing machines. The experimental results show that the diagnostic accuracy of the constructed model is 93.67%, which is a significant improvement over the other methods.
