作者:
He, Qing;Queenborough, Simon A.;Zhang, Yonghua;Wang, Weitao;Li, Buhang;...
期刊:
American Journal of Botany,2023年110(2):e16124 ISSN:0002-9122
通讯作者:
Chengjin Chu
作者机构:
[Tang, Hui; Wang, Weitao; Luo, Wenqi; He, Qing; Li, Buhang; Chu, Chengjin; Lin, Wei] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China.;[Tang, Hui; Wang, Weitao; Luo, Wenqi; He, Qing; Li, Buhang; Chu, Chengjin; Lin, Wei] Sun Yat Sen Univ, Sch Ecol, Guangzhou 510275, Peoples R China.;[Queenborough, Simon A.] Yale Univ, Yale Sch Environm, New Haven, CT 06511 USA.;[Zhang, Yonghua] Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325000, Peoples R China.;[Zhao, Kangning] Univ South China, Sch Architecture, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Chengjin Chu] S;State Key Laboratory of Biocontrol, School of Life Sciences and School of Ecology, Sun Yat-sen University, Guangzhou, 510275 China
摘要:
PREMISE: Understanding the drivers of the growth in long-lived woody trees is the key to predicting their responses to and maintaining their populations under global change. However, the role of tree sex and differential investment to reproduction are often not considered in models of individual tree growth, despite many gymnosperm and angiosperm species having separate male and female sexes. Thus, better models of tree growth should include tree sex and life stage along with the abiotic and biotic neighborhoods. METHODS: We used a sex-specific molecular marker to determine the sex of 2188 individual trees >1 cm DBH of the dioecious tree species Diospyros morrisiana in a 50-ha subtropical forest plot in China. We used long-term census data from about 300,000 trees, together with 625 soil samples and 2352 hemispherical photographs to characterize the spatially explicit biotic and abiotic neighborhoods. RESULTS: We found a male-biased effective sex ratio and a female-biased overall population sex ratio of D. morrisiana. No sex spatial segregation was detected for the overall population, mature, or immature trees. Immature trees grew faster than mature trees and females grew slower than males. Further, conspecific neighbors significantly decreased tree growth, while the abiotic neighborhood showed no significant effect. CONCLUSIONS: Our findings suggest that variation in resource allocation patterns within and across individual trees of different sexes and life-history stages should be more widely accounted for in models of tree growth. In addition, our study highlights the importance of sex-specific molecular markers for studying populations of long-lived dioecious tree species.
摘要:
Theranostic approaches with positron emission tomography/computed tomography (PET/CT) or PET/magnetic resonance imaging (PET/MRI) molecular imaging probes are being implemented clinically in prostate cancer (PCa) diagnosis and imaging-guided precision surgery. This review article provides a comprehensive summary of the rapidly expanding list of molecular imaging probes in this field, including their applications in early diagnosis of primary prostate lesions; detection of lymph node, skeletal and visceral metastases in biochemical relapsed patients; and intraoperative guidance for tumor margin detection and nerve preservation. Although each imaging probe shows preferred efficacy in some applications and limitations in others, the exploration and research efforts in this field will eventually lead to improved precision theranostics of PCa.
摘要:
The interaction between shale and various fluids is crucial as it modifies pore structures, which govern the effective development of shale gas and the geological storage of carbon dioxide in shale formations. In this study, samples from the Longmaxi Formation shale in Sichuan Basin of China were exposed to different fluids, including 6 MPa CO2, 12 MPa CO2, 6 MPa CO2+brine, and 12 MPa CO2+brine, at 45 degrees C for 100 days. Various methods, including X-ray diffraction (XRD), X-ray fluorescence (XRF), field-emission scanning electron microscopy (FESEM), and the low-pressure gas adsorption (N-2) test, were adopted to evaluate chemical and structural changes during the exposure process. After being treated with supercritical CO2+brine and subcritical CO2+brine, the shale underwent significant changes in its major element composition. The content of Ca, Al, and K in shale saturated with supercritical CO2+brine decreased from 13.00% to 10.34%, from 3.65% to 3.36%, and from 1.56% to 1.37%, respectively. Meanwhile, the content of Si and Na in the same shale increased slightly after saturation. The amount of quartz and dolomite increased, while the levels of clay and calcite slightly decreased. The surface of the shale sample became rougher and small bumps and cracks appeared after saturation with different fluids, as shown by the FESEM analysis results. Furthermore, the changes in both the total pore volume and pore size followed a similar pattern to the alterations in the specific surface areas. The highest level of variation occurred with the shale that was saturated with 12 MPa of CO2, indicating that gas pressure and CO2 phase state have a significant influence on the shale's pore structure. In addition, the distribution of pore sizes showed a bias towards larger sizes across all diameters; this suggests that the reaction resulted in a decrease in the number of micropores. This also highlights that the impact of varying fluid saturation was primarily focused on micropores and macropores. The results of this study provided experimental evidence to further test the mechanisms and permeability of geological storage of CO2 in organic-rich self-sourced shale.
摘要:
Ferroptosis is a novel type of cell death associated with iron accumulation and excessive lipid peroxidation. Elucidating the underlying molecular mechanisms of ferroptosis is intensively related to the development and treatment of multiple diseases, including musculoskeletal disorders. Moreover, in vitro and in vivo studies have shown the importance of oxidative stress in musculoskeletal conditions such as osteoporosis, osteoarthritis, rheumatoid arthritis, and osteosarcoma. Ferroptosis-derived clinical management of musculoskeletal diseases offers tremendous and attractive opportunities. Notably, ferroptosis agonists have been proven to enhance the sensitivity of osteosarcoma cells to conventional therapeutic strategies. In this review, we have mainly focused on the implications of ferroptosis regulation in the pathophysiology and therapeutic response of musculoskeletal disorders. Understanding roles of ferroptosis for controlling musculoskeletal diseases might provide directions for ferroptosis-driven therapies, which could be promising for the development of novel therapeutic strategies.
摘要:
In the ABL wind tunnel, three inverted umbrella-shaped membrane structure groups were subjected to simultaneous pressure measurement model test. Time series of the fluctuating wind pressure were collected from 24 different wind angles. Upon examining the correlation coefficient of the fluctuating wind pressure between the upper and lower surfaces at the same pressure tap, it was discovered that the fluctuating wind pressure of the upwind roof exhibited asynchronous changes, which is a characteristic of a negative correlation area and has detrimental effects on the structure's wind resistance. The local areas with non-Gaussian properties were evaluated by analyzing the probability density function of representative pressure taps. The mechanism behind non-Gaussian wind pressure was elucidated through considerations of the central limit theorem and spatial correlation of fluctuating wind pressure. This study introduces a quantitative classification criterion for identifying the non-Gaussian distribution area of fluctuating wind pressure, setting the threshold at an 80% cumulative probability for both skewness and kurtosis. The analysis showed that the steady airflow of the structure corresponds to the Gaussian distribution area, while the non-Gaussian distribution area is primarily concentrated at the front edge of the upwind roof and the rear edge of the downwind roof. To ascertain the peak factor for the non-Gaussian distribution, the improved peak factor method was utilized, providing insights into the range of peak factor in the non-Gaussian distribution area of this particular roof configuration. This information serves as a foundation for the wind resistance design of this structure.
作者机构:
[Xu, Ye; Liu, Rushi; Li, Xiaofeng; Zhong, Zhihong; Tan, Chufan; Li, Sang] Hunan Normal Univ, Sch Med, Lab Med Mol & Immunol Diagnost, Changsha 410013, Peoples R China.;[Qiu, Yilan; Qiu, YL] Hunan Normal Univ, Sch Life Sci, Changsha 410081, Peoples R China.;[Wu, Junwen] Nanya Middle Sch Changsha, Changsha 410129, Peoples R China.;[Feng, Bo; Feng, B] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Changsha 410008, Peoples R China.
通讯机构:
[Feng, B ] U;[Qiu, YL ] H;Hunan Normal Univ, Sch Life Sci, Changsha 410081, Peoples R China.;Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Changsha 410008, Peoples R China.
关键词:
Hepatitis E;HEV vaccine;Virus -like particles;The recombinant capsid protein p27
摘要:
HEV (Hepatitis E virus) is an infectious disease transmitted between humans and animals, which poses a severe threat to the biological safety and property throughout the world. The disease is especially severe in patients with potential liver cirrhosis and women during pregnancy. There is no specific and thorough HEV treatment at present. The development of hepatitis E virus vaccine is vital to the prevention of viral hepatitis worldwide. Since HEV cannot grow adequately in vitro, vaccine developed by devitalized virus particles does not work. Exploration of HEV-like structures is essential for the development of functional vaccines against HEV infection. ORF2 encodes the structural proteins of HEV, some of which can automatically assemble into virus-like particles (VLP) in this experiment, the recombinant capsid protein p27 was expressed in E. coli and the VLP formed by p27 was used to immunize mice. The results showed that the VLP formed by recom-binant P27 had similar particle size to that of HEV; the immune dose produced by p27 was positively correlated with the immune effect. Compared with other genetic engineering subunit vaccines, P27 protein has a better application prospect.
摘要:
Heavy metal pollution seriously threatens the environment and human health. The biosorption of heavy metals has attracted worldwide attention due to its cost-effectiveness and environmental friendliness. It is significant to develop biosorbents with excellent adsorption performance. Sphingopyxis is widely used in the removal of various organic pollutants, but its potential application in heavy metal adsorption has been largely overlooked. This study investigates the biosorption of U(VI) onto live and dead cells of a Sphingopyxis strain YF1. The effects of pH, contact time and initial ion concentration on U(VI) adsorption investigated, and kinetic and isothermal models were used to fit the adsorption results. The results show that under pH 3-6, the adsorption of U(VI) by YF1 live cells increased with the increase of the pH. Both the pseudo-first order and pseudo-second order models can satisfactorily interpret the adsorption by live and dead cells. Three isothermal adsorption models (Langmuir, Freundlich, and Sips) were used to fit the adsorption process. The adsorption of uranium by live and dead cells was best fitted by the Sips model. The maximal adsorption capacities of U(VI) by live and dead cells were 140.7 mg g(-1) and 205.7 mg g(-1), respectively. The mechanisms of U(VI) adsorption by Sphingopyxis sp. YF1 were revealed. Scanning electron microscopy and energy dispersive spectroscopy (SEM-EDS) show that U(VI) was deposited on the surface of the bacterial cells. Fourier-transform infrared spectroscopy (FTIR) shows that amine, hydroxyl, alkyl, amide I, amide II, phosphate, carboxylates and carboxylic acids were the major functional groups that are involved in U(VI) adsorption by live and dead cells. X-ray photoelectron spectroscopy (XPS) suggests that the main functional groups of live cells involved in adsorption were O = C-O, C-OH/C-O-C and N-C = O. This study indicates Sphingopyxis sp. YF1 is a high-efficiency U(VI)-adsorbing strain, promising to remove U(VI) from aquatic environment.
摘要:
Investigating the tectonostratigraphic evolution of Late Mesozoic basins in NE China and its surrounding areas offers great opportunities to restore the Late Mesozoic evolution of intraplate tectonic stress regime in East Asia and to uncover the relationships between intraplate deformation and plate margin tectonic processes. This study provides a comprehensive analysis of the stratigraphic and structural evolution of the basins in NE China and its surrounding areas during the Late Mesozoic. It is shown that these basins underwent significant extension and syn-rift subsidence during the Late Jurassic-Early Cretaceous. However, the long-lived extension was intermittent rather than continuous, and at least three pulses of short-lived episodic compressive deformation, that occurred at the latest Jurassic-earliest Cretaceous, middle Aptian (ca. 120 Ma), and late Aptian (ca. 115 Ma), could be recognized. The Late Jurassic-Early Cretaceous extension was terminated by a strong contractional event at the earliest Late Cretaceous. The frequent switches in regional tectonic stress regime between extension and contraction require a new tectonic interpretation, as proposed here. We suggest that during the Late Jurassic-Early Cretaceous, the continental collision and subsequent northeastward indentation of the Karakoram-Lhasa Block with the southern Asian margin probably activated a significant strike-slip fault system, propagating approximately from the collision zone to the eastern Asian margin, and drove East Asia into a lateral extrusion/escape tectonic setting. The activated strike-slip faulting probably triggered the postorogenic gravitational collapse of the thickened upper crust and the delamination of the lower crust and mantle lithosphere, resulting in regional extension and intense magmatism in East Asia during the Late Mesozoic. The intercurrent multiphase short-lived contractional events were likely associated with a series of continental collision events along Asian margins. The latest Jurassic-earliest Cretaceous contractional deformation probably resulted from the combined effects of the closure of the eastern Mongol-Okhotsk Ocean, the Kolyma-Omolon/Siberia collision, and the Karakoram-Lhasa/Southern Asia collision. The middle Aptian contractional deformation probably resulted from the Proto-Kurosegawa/Eastern Asia collision. The late Aptian contractional deformation probably resulted from the Chukotka/Kolyma-Omolon collision. The earliest Late Cretaceous contractional deformation probably resulted from the Okhotomorsk/Eastern Asia collision.
摘要:
Contrast-enhanced ultrasound (CEUS) is a dependable modality for the diagnosis of various clinical conditions. A judicious selection of ultrasound contrast agent (UCA) is imperative for optimizing imaging and improving diagnosis. Approved UCAs for imaging the majority of organs include SonoVue, a pure blood agent, and Sonazoid, which exhibits an additional Kupffer phase. Despite the fact that the two UCAs are increasingly being employed, there is a lack of comparative reviews between the two agents in different organs diseases. This review represents the first attempt to compare the two UCAs in non-hepatic organs, primarily including breast, thyroid, pancreas, and spleen diseases. Through comparative analysis, this review provides a comprehensive and objective evaluation of the performance characteristics of SonoVue and Sonazoid, with the aim of offering valuable guidance for the clinical application of CEUS. Overall, further clinical evidences are required to compare and contrast the dissimilarities between the two UCAs in non-hepatic organs, enabling clinicians to make an appropriate selection based on actual clinical applications.
摘要:
It is a well-known phenomenon that natural products can serve as powerful drug leads to generate new molecular entities with novel therapeutic utility. Evodiamine (Evo), a major alkaloid component in traditional Chinese medicine Evodiae Fructus, is considered a desirable lead scaffold as its multifunctional pharmacological properties. Although natural Evo has suboptimal biological activity, poor pharmacokinetics, low water solubility, and chemical instability, medicinal chemists have succeeded in producing synthetic analogs that overshadow the deficiency of Evo in terms of further clinical application. Recently, several reviews on the synthesis, structural modification, mechanism pharmacological actions, structure-activity relationship (SAR) of Evo have been published, while few reviews that incorporates intensive structural basis and extensive SAR are reported. The purpose of this article is to review the structural basis, anti-cancer activities, and mechanisms of Evo and its derivatives. Emphasis will be placed on the optimizing strategies to improve the anticancer activities, such as structural modifications, pharmacophore combination and drug delivery systems. The current review would benefit further structural modifications of Evo to discover novel anticancer drugs.
通讯机构:
[Liu, ZG; Li, C ; Lin, YH ] X;Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res, Xiamen 361005, Peoples R China.;Xiamen Univ, Eye Inst, Sch Med, Fujian Prov Key Lab Ophthalmol & Visual Sci & Ocul, Xiamen 361102, Peoples R China.;Xiamen Univ, Affiliated Xiamen Eye Ctr, Sch Med, Xiamen 361102, Peoples R China.;Xiamen Univ, Natl Inst Data Sci Hlth & Med, Xiamen 361102, Peoples R China.
关键词:
Chemical ocular burn;Corneal diseases;Halogen;Metal–organic frameworks nanozyme;Superoxide dismutase
摘要:
Metal-organic frameworks (MOFs) have attracted significant research interest in biomimetic catalysis. However, the modulation of the activity of MOFs by precisely tuning the coordination of metal nodes is still a significant challenge. Inspired by metalloenzymes with well-defined coordination structures, a series of MOFs containing halogen-coordinated copper nodes (Cu-X MOFs, X = Cl, Br, I) are employed to elucidate their structure-activity relationship. Intriguingly, experimental and theoretical results strongly support that precisely tuning the coordination of halogen atoms directly regulates the enzyme-like activities of Cu-X MOFs by influencing the spatial configuration and electronic structure of the Cu active center. The optimal Cu-Cl MOF exhibits excellent superoxide dismutase-like activity with a specific activity one order of magnitude higher than the reported Cu-based nanozymes. More importantly, by performing enzyme-mimicking catalysis, the Cu-Cl MOF nanozyme can significantly scavenge reactive oxygen species and alleviate oxidative stress, thus effectively relieving ocular chemical burns. Mechanistically, the antioxidant and antiapoptotic properties of Cu-Cl MOF are achieved by regulating the NRF2 and JNK or P38 MAPK pathways. Our work provides a novel way to refine MOF nanozymes by directly engineering the coordination microenvironment and, more significantly, demonstrating their potential therapeutic effect in ophthalmic disease.
摘要:
Human papillomavirus (HPV) E7 protein as an important viral factor was involved in the progression of cervical cancer by mediating the cellular signaling pathways. Daxx (Death domain-associated protein) can interact with a variety of proteins to affect the viral infection process. However, the interaction and its related function between HPV16 E7 and Daxx have not been adequately investigated. Here, it was found that HPV16 E7 can interact with Daxx in HeLa or C33A cells by co-immunoprecipitation. HPV16 E7 protein treatment can up-regulate Daxx protein expression, while the interference in Daxx expression and the agonists for JNK can both reduce the antagonistic effects of HPV16 E7 on TNF-α-induced apoptosis, suggesting that Daxx/JNK pathway may be involved in the anti-apoptotic activity of HPV16 E7.
摘要:
Most traditional infrared and visible image fusion methods often ignore weak texture details, especially for the low-light visible images in which the weak details are easily drowned due to noise or ill-illumination. To ad -dress this problem, we propose a novel infrared and low-light visible image fusion method from the perspective of low-light visible image enhancement, weak feature extraction strategy and detail preserved fusion rules. By combining both local and global contrast enhancements, an adaptive light adjustment algorithm is proposed to improve the brightness and texture details of low-light visible images. In addition, we design a hybrid multiscale decomposition model based on guided filters (GFs) and side window guided filters (SWGFs) to decompose the source images into the base layer, large-scale detail layers and small-scale detail layers. The three layers reflects the background, large edge structures and weak texture details of source images respectively. Subsequently, the visual saliency retention, normalized arctan function, and edge preservation-based consistency verification are applied to highlight salient targets and retain weak details for three layers fusion. Qualitative and quantitative ex-perimental results on publicly available datasets prove the superiority of our method over state-of-the-art methods in terms of highlight salient targets, avoiding edge blurring, and retaining weak details.
摘要:
Saxitoxin (STX) is a neurotoxin produced by certain toxic cyanobacteria and dinoflagellates. It is biochemically synthesized by the stepwise involvement of eight core enzymes encoded in sxt genes. Of them, sxtB, a cytidine deaminase (CDA)-like enzyme, may participate in the third step (after sxtA and sxtG) in the toxin synthesis pathway; however, it is insufficiently elucidated in toxic dinoflagellates. In the present study we determined a novel sxtB gene from the STX-producing dinoflagellate Alexandrium catenella, characterized structural motifs and phylogenetic origin, and evaluated transcriptional responses of the gene under different temperatures. cDNA of the AcsxtB was 1,146 bp in length from dinoflagellate spliced leader (Dino-SL) to poly (A) tail. It comprised a 972 bp open reading frame, encoding a 323 aa protein with a molecular weight of 34.20 kDa and isoelectric point of 6.36. In addition, we identified a putative mitochondrial transfer peptide and no introns in genomic coding regions. The AcsxtB was phylogenetically close to toxic cyanobacteria, but distant to non-toxic dinoflagellates. The transcription levels of AcsxtB were significantly up-regulated when cultured at 16 degrees C (3.36-fold) and exposed to cold stress (20 -> 12 degrees C; 1.88-fold, 20 -> 16 degrees C; 2.07-fold), which showed a high correlation with increased STX contents. These suggest that the sxtB should participate in STX synthesis, but its involvement may be low or regulated in very early stages before 72 h. This is the first report on sxtB characterization and transcriptional regulation with relevance to STX production in the toxic dinoflagellates.
期刊:
Chemical Engineering Journal,2023年452:139228 ISSN:1385-8947
通讯作者:
Li, Zhenye(lizhenye@usc.edu.cn)
作者机构:
[Li, Zhenye] Univ South China, Coll Mech Engn, Hengyang 421001, Peoples R China.;[Ying, Lei; Peng, Feng] South China Univ Technol, Inst Polymer Optoelect Mat & Devices, State Key Lab Luminescent Mat & Devices, Guangzhou 510640, Peoples R China.;[Liang, Yingfang] Univ South China, Hengyang 421001, Peoples R China.;[Li, Zhenye; Chen, Lin; Chen, Jian] Hunan Dahe New Mat Co Ltd, Hengyang 421001, Peoples R China.
通讯机构:
[Zhenye Li] C;[Lei Ying] I;College of Mechanical Engineering, University of South China, Hengyang 421001, China<&wdkj&>Hunan Dahe New Materials Co., Ltd., Hengyang 421001, China<&wdkj&>Institute of Polymer Optoelectronic Materials and Devices, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, China
关键词:
All-polymer solar cells;Non-halogenated solvent;Polymer doping;Stability
作者机构:
[Zhou, Jing; Yao, Xu; Xiang, Yi-Jun; Liu, Shun] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Key Lab Tumor Microenvironm Respons Dru, Hengyang 421001, Peoples R China.;[Lin, Jin-Hong; Xiao, Ji-Chang; Xiang, Yi-Jun] Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Organ Chem, Key Lab Organofluorine Chem, Shanghai 200032, Peoples R China.;[Lin, Jin-Hong] Shanghai Univ, Innovat Drug Res Ctr, Dept Chem, Shanghai 200444, Peoples R China.
通讯机构:
[Jin-Hong Lin; Ji-Chang Xiao] K;[Xu Yao] I;Institute of Pharmacy and Pharmacology, Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research, University of South China, 421001 Hengyang, PR China<&wdkj&>Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, 200032 Shanghai, PR China<&wdkj&>Key Laboratory of Organofluorine Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences, 345 Lingling Road, 200032 Shanghai, PR China<&wdkj&>Department of Chemistry, Innovative Drug Research Center, Shanghai University, 200444 Shanghai, PR China
摘要:
Described here is the R3P/ICH2CH2I-promoted dehydroxylative sulfonylation of alcohols with a variety of sulfinates. In contrast to previous dehydroxylative sulfonylation methods, which are usually limited to active alcohols, such as benzyl, allyl, and propargyl alcohols, our protocol can be extended to both active and inactive alcohols (alkyl alcohols). Various sulfonyl groups can be incorporated, such as CF3SO2 and HCF2SO2, which are fluorinated groups of interest in pharmaceutical chemistry and the installation of which has received increasing attention. Notably, all reagents are cheap and widely available, and moderate to high yields were obtained within 15 min of reaction time.
期刊:
PATHOLOGY RESEARCH AND PRACTICE,2023年248:154634 ISSN:0344-0338
通讯作者:
Cao, JY;Dai, T
作者机构:
[Cao, Jingying] Cent South Univ, Xiangya Hosp 3, Dept Med Clin Lab, Changsha 410013, Hunan, Peoples R China.;[Cao, Renxian] Univ South China, Affiliated Hosp 1, Inst Clin Med, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Liu, Yiqi] Univ South China, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China.;[Dai, Tao] Cent South Univ, Xiangya Sch Med, Hunan Canc Hosp, Affiliated Canc Hosp,Dept Urol, Changsha 410013, Hunan, Peoples R China.;[Cao, Jingying; Cao, JY] Cent South Univ, Xiangya Hosp 3, Dept Med Clin Lab, 138 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Dai, T ; Cao, JY ] C;Cent South Univ, Xiangya Hosp 3, Dept Med Clin Lab, 138 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.;Cent South Univ, Xiangya Sch Med, Hunan Canc Hosp, Affiliated Canc Hosp,Dept Urol, 283 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.
关键词:
AKT;CPNE1;Glycolysis;PI3K;Triple-negative breast cancer
摘要:
CPNE1 regulates multiple signaling pathways and can stimulate cell proliferation and differentiation by activating the AKT-mTOR signaling pathway. In addition, CPNE1 is associated with various cancers; however, its role in breast cancer, particularly in TNBC, has not been fully elucidated. Our study aimed to reveal the impact of the CPNE1/PI3K/AKT/HIF-1α axis on TNBC. We first measured the expression of CPNE1 in the tumor tissues of TNBC patients and examined its prognostic value. Subsequently, we used sh-CPNE1 and overexpression vectors to transfect TNBC cell lines and analyzed cell viability, migration, and invasive abilities using colony formation and CCK-8 assays. Metabolites were analyzed through metabolomics. We found that higher expression of CPNE1 predicted poor prognosis in TNBC patients. Knockdown of CPNE1 reduced the viability, migration, invasion, and proliferation capabilities of TNBC cells. Furthermore, metabolomics analysis showed that glucose metabolism was the most dominant pathway, and knockdown of CPNE1 significantly limited the glycolytic activity of TNBC cells. We verified these conclusions in mouse models. Additionally, we overexpressed CPNE1 and treated TNBC cell lines with a PI3K inhibitor (LY294002). The results indicated that CPNE1 promoted aerobic glycolysis in TNBC cells through the PI3K/AKT/HIF-1α signaling pathway. This suggests that CPNE1 regulates cell glycolysis and participates in the development of TNBC. Our study may provide a new therapeutic target for TNBC treatment.
通讯机构:
[Liu, K ] U;Univ South China, Pediat Acad, Hunan Childrens Hosp, Dept Pediat Orthoped, 86 Ziyuan Rd, Changsha 410000, Hunan, Peoples R China.;Hunan Prov Key Lab Pediat Orthoped, Changsha 410008, Hunan, Peoples R China.
关键词:
Developmental Dysplasia of the hip;Anterior Approach;Open reduction;Hip Joint;Surgical Treatment
摘要:
OBJECTIVES: To investigate the clinical efficacy and safety of open reduction through anterior minimally invasive approach in the treatment of children with developmental dysplasia of the hip. METHOD: A total of 23 patients (25 hips) less than 2 years with developmental dysplasia of the hip treated by open reduction through anterior minimally invasive approach were treated in our hospital from August 2016 to March 2019. Through the anterior minimally invasive approach, we enter from the gap between sartorius muscle and tensor fasciae lata without cutting off rectus femoris muscle, which can effectively expose the joint capsule and reduce the damage to medial blood vessels and nerves. The operation time, incision length, intraoperative bleeding, hospital stay and surgical complications were observed. The progression of developmental dysplasia of the hip and avascular necrosis of the femoral head were evaluated by imaging examination. RESULT: All patients were performed with follow-up visit for an average of 22 months. The average incision length was 2.5cm, the average operation time was 26min, the average intraoperative bleeding was 12ml, and the average hospital stay was 4.9 days. All patients received concentric reduction immediately after operation, and no re-dislocation occurred. At the last follow-up visit, the acetabular index was (25.8 ± 6.4°). During the follow-up visit, X-ray showed avascular necrosis of the femoral head in 4 hips (16%). CONCLUSION: open reduction through anterior minimally invasive approach can achieve good clinical effect in the treatment of infantile developmental dysplasia of the hip.