摘要:
To evaluate the immune protective efficacy of Chlamydia psittaci plasmid protein CPSIT_p7 and to analyze the potential mechanisms.In the current study,recombinant CPSIT_p7 protein was used to vaccinate BALB/c mice utilizing complete FA (FCA) and incomplete FA (FIA) as adjuvants.Adjuvants alone or PBS formulated with the same adjuvants were used as negative controls,respectively.
期刊:
annals of the rheumatic diseases,2021年80(SUPPL-1):1057-1057 ISSN:0003-4967
作者机构:
[Li, T. W.; Huang, Q.; Sun, S.; Ji, Z.; Liu, Y.; Huang, Y.; Deng, W.] Guangdong Second Prov Gen Hosp, Dept Rheumatol & Immunol, Guangzhou, Peoples R China.;[Liu, Y.] Univ South China, Guangdong Prov Gen Hosp 2, Guangzhou, Peoples R China.
会议名称:
Annual European Congress of Rheumatology (EULAR)
会议时间:
JUN 02-05, 2021
会议地点:
ELECTR NETWORK
摘要:
Background: Exosomes in synovial fluid (SF) has a close relationship with the pathogenesis of rheumatiod arthritis. As a complex biological fluid, SF presents challenges for exosomes isolation using standard methods, such as ExoquickTM kit and ultracentrifugation. Objectives: The study aims to compared the quality of exosomes separated by ExoquickTM kit (TM), ExoquickTM kit+ExoquickTC kit (TM-TC), ultracentrifugation (UC) and TM-TC+UC(TM-TC-UC) from SF. Methods: Exosomes was separated by TM, TM-TC, UC and TM-TC-UC respectively. The size and concentrations of exosomes were detected by high sensitivity flow cytometry for nanoparticle analysis. Total protein and RNA were extracted from exosomes. SDS-PAGE was used to detect the protein distribution of exosomes. Western blot was used to examine the level of albumin and exosomes marker (TSG101 and CD81). Results: There was no statistic difference in the diameters of exosomes separated by the four methods. The concentrations of exosomes in TM, TM-TC, TM-TC-UC and UC were (5.65±0.93), (3.02±1.19), (1.67±0.25) and (4.61±0.73) *109Particles/mL. The protein concentrations of exosomes separated by the four methods were consistent with the concentrations of exosomes. SDS-PAGE showed that the protein distribution of exosomes separated by the four methods were different. Low levels of albumin were detected in TM-TC and TM-TC-UC, while high levels of albumin in TM and UC. Total RNA concentrations from exosomes in TM-TC was higher than other groups. Conclusion: TM-TC can be used to obtain higher quality exosomes from SF for the study of exosome-enriched components. References: [1]Helwa I, et al, A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents. PloS one, 2017. 12(1): p. e0170628-e0170628. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Disclosure of Interests: None declared
摘要:
目的 评价一种新型翼形钛网在退行性颈椎病前路手术中应用的临床效果。方法2009年7月至2015年7月应用该椎间融合器治疗退变性颈椎病58例,男34例,女24例;颈椎间盘突出(herniation of cervical disc,CDH)44例,后纵韧带钙化(ossification of posterior longitudinal ligament,OPLL)14例;单节段病变39例,双节段病变19例。