摘要：
低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)升高是冠脉粥样硬化性心脏病的重要危险因素,糖代谢异常患者由于胰岛素生物调节障碍,进一步加重脂质代谢紊乱,从而进一步升高LDL-C水平.最新ESC/EAS指南提出应高度重视糖代谢异常患者LDL-C达标率.最近一项对2674名糖尿病患者的研究调查显示LDL-C达标率仅为13.5％；因此糖代谢异常患者更应积极尽早降低LDL-C预防冠心病.
期刊：
annals of the rheumatic diseases,2021年80(SUPPL-1):1057-1057 ISSN：0003-4967
作者机构：
[Li, T. W.; Huang, Q.; Sun, S.; Ji, Z.; Liu, Y.; Huang, Y.; Deng, W.] Guangdong Second Prov Gen Hosp, Dept Rheumatol & Immunol, Guangzhou, Peoples R China.;[Liu, Y.] Univ South China, Guangdong Prov Gen Hosp 2, Guangzhou, Peoples R China.
会议名称：
Annual European Congress of Rheumatology (EULAR)
会议时间：
JUN 02-05, 2021
会议地点：
ELECTR NETWORK
摘要：
Background: Exosomes in synovial fluid (SF) has a close relationship with the pathogenesis of rheumatiod arthritis. As a complex biological fluid, SF presents challenges for exosomes isolation using standard methods, such as ExoquickTM kit and ultracentrifugation. Objectives: The study aims to compared the quality of exosomes separated by ExoquickTM kit (TM), ExoquickTM kit+ExoquickTC kit (TM-TC), ultracentrifugation (UC) and TM-TC+UC(TM-TC-UC) from SF. Methods: Exosomes was separated by TM, TM-TC, UC and TM-TC-UC respectively. The size and concentrations of exosomes were detected by high sensitivity flow cytometry for nanoparticle analysis. Total protein and RNA were extracted from exosomes. SDS-PAGE was used to detect the protein distribution of exosomes. Western blot was used to examine the level of albumin and exosomes marker (TSG101 and CD81). Results: There was no statistic difference in the diameters of exosomes separated by the four methods. The concentrations of exosomes in TM, TM-TC, TM-TC-UC and UC were (5.65±0.93), (3.02±1.19), (1.67±0.25) and (4.61±0.73) *109Particles/mL. The protein concentrations of exosomes separated by the four methods were consistent with the concentrations of exosomes. SDS-PAGE showed that the protein distribution of exosomes separated by the four methods were different. Low levels of albumin were detected in TM-TC and TM-TC-UC, while high levels of albumin in TM and UC. Total RNA concentrations from exosomes in TM-TC was higher than other groups. Conclusion: TM-TC can be used to obtain higher quality exosomes from SF for the study of exosome-enriched components. References: [1]Helwa I, et al, A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents. PloS one, 2017. 12(1): p. e0170628-e0170628. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Download figure Open in new tab Download powerpoint Figure 1. A: SDS-PAGE showed the protein distribution of exosomes; B: the detection of albumin, TSG101 and CD81 by western blot. Disclosure of Interests: None declared
作者机构：
University of South China, Hunan, China;Hunan Medical Big Data International Sci.&Tech. Innovation Cooperation Base, Hunan, China;[Ma X.; Liu Y.; Ouyang C.] University of South China, Hunan, China, Hunan Medical Big Data International Sci.&Tech. Innovation Cooperation Base, Hunan, China
会议名称：
5th China Conference on Knowledge Graph, and Semantic Computing, CCKS 2020
会议时间：
12 November 2020 through 15 November 2020
关键词：
Event detection;Graph convolutional networks;Hybrid representation;Syntactic information
作者机构：
[Ning Z.] University of South China, Hengyang, China;[Si W.; Liao X.] Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China;[Tang Y.] University of South China, Hengyang, China, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
会议名称：
11th International Conference on Electronics, Communications and Networks, CECNet 2021
