通讯作者：
Manbo Cai<&wdkj&>Xiaoming Xie<&wdkj&>Yutian Zou<&wdkj&>Manbo Cai Manbo Cai Manbo Cai<&wdkj&>Xiaoming Xie Xiaoming Xie Xiaoming Xie<&wdkj&>Yutian Zou Yutian Zou Yutian Zou
作者机构：
[Hongbo Zhu; Manbo Cai; Hongbo Zhu Hongbo Zhu Hongbo Zhu; Manbo Cai Manbo Cai Manbo Cai] The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China;[Jindong Xie; Xinpei Deng; Yi Xie; Peng Liu; Wei Deng; Li Ning; Yuhui Tang; Yuying Sun; Hailin Tang; Xiaoming Xie; Yutian Zou; Jindong Xie Jindong Xie Jindong Xie; Xinpei Deng Xinpei Deng Xinpei Deng; Yi Xie Yi Xie Yi Xie; Peng Liu Peng Liu Peng Liu; Wei Deng Wei Deng Wei Deng; Li Ning Li Ning Li Ning; Yuhui Tang Yuhui Tang Yuhui Tang; Yuying Sun Yuying Sun Yuying Sun; Hailin Tang Hailin Tang Hailin Tang; Xiaoming Xie Xiaoming Xie Xiaoming Xie; Yutian Zou Yutian Zou Yutian Zou] State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong, China
通讯机构：
[Manbo Cai; Manbo Cai Manbo Cai Manbo Cai] T;[Xiaoming Xie; Yutian Zou; Xiaoming Xie Xiaoming Xie Xiaoming Xie; Yutian Zou Yutian Zou Yutian Zou] S;The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, China<&wdkj&>State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong, China
摘要：
Flowchart of our study. By utilizing multi‐omics pan‐cancer cohorts, our study firstly offers a pan‐cancer blueprint of the molecular and clinical characteristics of disulfidptosis regulators, as wells as disulfidptosis activity, which lay a solid foundation for the disulfidptosis‐targeting strategy in precision cancer treatment. Abstract Disruption of disulfide homeostasis during biological processes can have fatal consequences. Excess disulfides induce cell death in a novel manner, termed as “disulfidptosis.” However, the specific mechanism of disulfidptosis has not yet been elucidated. To determine the cancer types sensitive to disulfidptosis and outline the corresponding treatment strategies, we firstly investigated the crucial functions of disulfidptosis regulators pan‐cancer at multi‐omics levels. We found that different tumor types expressed dysregulated levels of disulfidptosis regulators, most of which had an impact on tumor prognosis. Moreover, we calculated the disulfidptosis activity score in tumors and validated it using multiple independent datasets. Additionally, we found that disulfidptosis activity was correlated with classic biological processes and pathways in various cancers. Disulfidptosis activity was also associated with tumor immune characteristics and could predict immunotherapy outcomes. Notably, the disulfidptosis regulator, glycogen synthase 1 (GYS1), was identified as a promising target for triple‐negative breast cancer and validated via in vitro and in vivo experiments. In conclusion, our study elucidated the complex molecular phenotypes and clinicopathological correlations of disulfidptosis regulators in tumors, laying a solid foundation for the development of disulfidptosis‐targeting strategies for cancer treatment.
关键词：
Germanium;Tartaric acid;Selective separation;Column separation;D201 x 7 resin
摘要：
Germanium (Ge) exhibits excellent optical and electrical properties. Due to the low reserves of Ge and lack of independent mineral resources, zinc refinery residues have become an important source of Ge. In this work, a D201 x 7 resin has been employed in the selective separation of Ge from a sulfuric solution using tartaric acid as a complexing agent. In the sulfuric acid system, Ge(OH)4 was converted to an anionic complex [GeO2(OH)2C4H4O4]2- at a 1:1 tartaric acid to Ge molar ratio. The anion exchange D201 x 7 resin adsorbed the Ge tartrate anionic complex. The adsorption process exhibited pseudo-second-order kinetic behavior and adhered to the Langmuir isothermal adsorption model. The maximum adsorption capacity of D201 x 7 resin for Ge was 214 mg g-1. Selective Ge uptake and purification could proceed by column separation The results showed that enrichment factor was 74.7, and the desorption concentration of Ge was increased from 500 mg L-1 to 36.6 g L-1. The D201 x 7 resin exhibited high selectivity, excellent adsorption performance and good stability, making it a viable candidate in industrial applications.
期刊：
Journal of Hazardous Materials,2024年468:133865 ISSN：0304-3894
通讯作者：
Ye, Yongjun
作者机构：
[Xia, Ming; Ye, Yongjun; Zhou, Ning] School of Resources Environment and Safety Engineering, University of South China, Hengyang 421001, China;[Ye, Yongjun] Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China. Electronic address: yongjunye@163.com
通讯机构：
[Ye, Yongjun] K;Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang 421001, China. Electronic address:
摘要：
Measurements of radon exhalation rate using traditional methods can be affected by back-diffusion or differential pressure in the accumulation chamber, resulting in deviations between the measured and the true values. To obtain an accurate radon exhalation rate for evaluation of radon-risk regions, two novel approaches of measurements based on traditional methods were proposed. Repeated experiments were implemented on a self-designed stainless cylindrical vessel filled with uranium tailings sand. The measured radon exhalation rates on average were 0.51±0.02 and 0.52±0.02Bqm(-2) s(-1) for the two proposed methods, with 0.02% and 0.04%, respectively, deviations from the theoretical value. In addition, numerical techniques were employed to interpret the defects of traditional methods and mechanisms of proposed approaches to measure accurate values. Two novel approaches have significantly reduced the impact of back diffusion and differential pressure inside the chamber and consumed less time.
摘要：
This paper presents a theoretical solution for bond-slip behavior of SRC interface based on experiment, numerical simulation, and theoretical derivation. Push-out tests of five specimens were firstly carried out, based on which, the simplified bond-slip model was proposed. The specimen was fabricated using the Q345H-shape steel (400 mm x 200 mmx13 mm x 8 mm) and C50 concrete (350 mm x 600 mm) with a bonding length of 500 mm. A FE model was established based on the bond-slip model to analyze the nonlinear bonding stress that are difficult to obtain from the experiment. The research shows that although the bonding stress in the elastic stage is not uniformly distributed, it is basically uniformly distributed when the ultimate bearing capacity is reached. It proves that assuming the average interfacial bonding stress under ultimate load to replace the maximum bonding stress is reasonable, which is crucial for designer to estimate interface bearing capacity and maximum bonding stress. Moreover, five different stages were obtained by the FE model analysis and the theoretical equations of bonding stress nonlinear distribution were obtained based on the boundary condition of each stage. The theoretical solution clearly showed the relationship between interface nonlinear stress distribution and influencing parameters including the bond area, elasticity modulus of material, section area of specimen, bond-slip constitutive model, and the external load. Based on the theoretical model, the influences of different parameters can be directly obtained without conducting further experiments and establishing different FE models.
作者机构：
[Xia, Qingrong; Chen, Zhiyi] Institute of Medical Imaging, University of South China, Hengyang 421001, China;[Xia, Qingrong] Affiliated Nanhua Hospital, University of South China, Hengyang 421002, China;[Lin, Riqiang; Shu, Chengyou; Xia, Qingrong; Wang, Xiatian; Xie, Zhihua; Zhang, Jinke; Lv, Shengmiao; Xu, Haoxing; Gong, Xiaojing] Research Center for Biomedical Optics and Molecular Imaging, Key Laboratory of Biomedical Imaging Science and System, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China;[Lin, Riqiang; Shu, Chengyou; Xia, Qingrong; Wang, Xiatian; Xie, Zhihua; Zhang, Jinke; Lv, Shengmiao; Xu, Haoxing; Gong, Xiaojing] Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China;[Lin, Riqiang] Department of Electrical and Electronic Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China
通讯机构：
[Zhiyi Chen] I;Institute of Medical Imaging, University of South China, Hengyang 421001, China<&wdkj&>Institution of Medical Imaging, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha 410000, China
摘要：
The endometrium microvessel system, responsible for supplying oxygen and nutrients to the embryo, holds significant importance in evaluating endometrial receptivity (ER). Visualizing this system directly can significantly enhance ER evaluation. Currently, clinical methods like Narrow-band hysteroscopy and Color Doppler ultrasound are commonly used for uterine blood vessel examination, but they have limitations in depth or resolution. Endoscopic Photoacoustic Imaging (PAE) has proven effective in visualizing microvessels in the digestive tract, while its adaptation to uterine imaging faces challenges due to the uterus's unique physiological characteristics. This paper for the first time that uses high-resolution PAE in vivo to capture a comprehensive network of endometrial microvessels non-invasively. Followed by continuous observation and quantitative analysis in the endometrial injury model, we further corroborated that PAE detection of endometrial microvessels stands as a valuable indicator for evaluating ER. The PAE system showcases its promising potential for integration into reproductive health assessments.
摘要：
Sleep deprivation (SD) is a global public health burden, and has a detrimental role in the nervous system. Retina is an important part of the central nervous system; however, whether SD affects retinal structures and functions remains largely unknown. Herein, chronic SD mouse model indicated that loss of sleep for 4 months could result in reductions in the visual functions, but without obvious morphologic changes of the retina. Ultrastructural analysis by transmission electron microscope revealed the deterioration of mitochondria, which was accompanied with the decrease of multiple mitochondrial proteins in the retina. Mechanistically, oxidative stress was provoked by chronic SD, which could be ameliorated after rest, and thus restore retinal homeostasis. Moreover, the supplementation of two antioxidants, α-lipoic acid and N-acetyl-l-cysteine, could reduce retinal reactive oxygen species, repair damaged mitochondria, and, as a result, improve the retinal functions. Overall, this work demonstrated the essential roles of sleep in maintaining the integrity and health of the retina. More importantly, it points towards supplementation of antioxidants as an effective intervention strategy for people experiencing sleep shortages.
摘要：
ATP-binding cassette protein A1 (ABCA1) is a key protein in the transport of intracellular cholesterol to the extracellular and plays an important role in reduc-ing cholesterol accumulation in surrounding tissues. Bibliometric analysis refers to the cross-science of quan-titative analysis of a variety of documents by mathemati-cal and statistical methods. It combines an analysis of structural and temporal patterns in scholarly publica-tions with a description of topic concentration and types of uncertainty. This paper analyzes the history, hotspot, and development trend of ABCA1 through bibliometrics. It will provide readers with the research status and development trend of ABCA1 and help the hot research in this field explore new research directions. After screening, the research on ABCA1 is still in a hot phase in the past 20 years. ABCA1 is emerging in previously unrelated disciplines such as cancer. There were 551 key-words and 6888 breakout citations counted by CiteSpace. The relationship between cancer and cardiovascular dis-ease has been linked by ABCA1. This review will guide readers who are not familiar with ABCA1 research to quickly understand the development process of ABCA1 and provide researchers with a possible future research focus on ABCA1. (Curr Probl Cardiol 2024;49:102036.)
期刊：
Biological Trace Element Research,2024年 ISSN：0163-4984
通讯作者：
Liao, Hongqing;Peng, Cuiying
作者机构：
[Wang, Die; Zhou, Cuilan; Yin, Dan; Mao, Rui; Yu, Ping; Liu, Jun; Li, Suyun] Department of Cell Biology and Genetics, Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province, Department of Education, Key Laboratory of Hengyang City On Biological Toxicology and Ecological Restoration, Key Laboratory of Typical Environmental Pollution and Health Hazards, University of South China, Hengyang, 421001, Hunan, China;[Nie, Yulin] Department of Obstetrics and Gynecology, the Second Affiliated Hospital, University of South China, 30# Jiefang Road, Hengyang, 421001, Hunan, China;[Liao, Hongqing] Department of Obstetrics and Gynecology, the Second Affiliated Hospital, University of South China, 30# Jiefang Road, Hengyang, 421001, Hunan, China. 17175979@qq.com;[Peng, Cuiying] Department of Cell Biology and Genetics, Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province, Department of Education, Key Laboratory of Hengyang City On Biological Toxicology and Ecological Restoration, Key Laboratory of Typical Environmental Pollution and Health Hazards, University of South China, Hengyang, 421001, Hunan, China. pengcuiying2004@126.com
通讯机构：
[Peng, Cuiying; Liao, Hongqing] D;Department of Obstetrics and Gynecology, the Second Affiliated Hospital, University of South China, 30# Jiefang Road, Hengyang, 421001, Hunan, China.;Department of Cell Biology and Genetics, Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, Key Laboratory of Ecological Environment and Critical Human Diseases Prevention of Hunan Province, Department of Education, Key Laboratory of Hengyang City On Biological Toxicology and Ecological Restoration, Key Laboratory of Typical Environmental Pollution and Health Hazards, University of South China, Hengyang, 421001, Hunan, China.
关键词：
ART outcomes;Plasma metal levels;Polycystic ovary syndrome;in vitro fertilization
摘要：
The objective of this study is to explore the correlation of metal levels with assisted reproductive technology (ART) outcomes in polycystic ovary syndrome (PCOS) patients. The individuals were recruited who met the research criteria, only tubal factor or male infertility served as the control group (n = 40) and patient group was PCOS patients (n = 35). Individuals (n = 75) were divided into PCOS group (n = 35) and control group (n = 40). The normal body mass index (BMI) group (control) includes women with BMI < 25kg/m(2) in PCOS group (n = 24) and control group (n = 33), and BMI ≥ 25kg/m(2) in PCOS group (n = 11) and control group (n = 7). We performed an analysis of insulin resistance (IR) (n = 15) group and without insulin resistance (NIR) group (n = 20) in PCOS patient and control patients. Comparing difference demographic data, ART outcomes and the metal levels in every group respectively, the correlation of metal levels and ART outcomes in control participants and PCOS patients were analyzed by the Spearman correlation analysis, and multiple linear regression model was used to examine the association between the concentration of 19 metals and ART outcomes in PCOS group and control group. Plasma manganese (Mn), titanium (Ti), sodium (Na), magnesium (Mg), copper (Cu), calcium (Ca)/Mg ratio, and Cu/zinc (Zn) ratio levels in PCOS patients were higher than that in control, while Zn and Ca levels were lower in PCOS patients than that in control. The Mg levels had a positive connection with the number of eggs recovered, and the iron (Fe) levels were positively associated with the number of transplanted embryos in PCOS-IR. In PCOS-NIR, Mn levels positively correlated with the number of follicles and the number of good embryos. Silver (Ag) levels were negatively correlated with the number of follicles, and aluminum (Al) levels were negatively related with the normal fertilization and the number of good embryos. The Spearman analysis in PCOS-BMI ≥ 25 group exhibited that nickel (Ni) levels were negatively associated with the number of follicles. The plasma metal levels seem to affect the clinical manifestations and in vitro fertilization outcomes in assisted reproduction.
作者机构：
[Song, Wanying; Liu, Keli; Liu, Chunqiao; Lin, Haotian; Yang, Liu; Liang, Juanran; Zou, Bin; Li, Jing Jing; Peng, Peiyao; Wei, Lai; Peng, Shanzhen; Chen, Tingting; Zeng, Lei; Xue, Wei; Guo, Shixin; Liang, Qiaoxing; Li, Ye; Li, Wenxuan; Zhang, Zhihui] State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China;[Wen, Xiaofeng] School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510000, China;[Shang, Haitao] Precision Medicine Institute, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China;[Zhang, Zhihui] Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin 300384, China;[Li, Wenxuan] Department of Biostatistics, Yale School of Public Health, New Haven, CT 06510, USA
摘要：
The Crumbs homolog 1 (CRB1) gene is associated with retinal degeneration, most commonly Leber congenital amaurosis (LCA) and retinitis pigmentosa (RP). Here, we demonstrate that murine retinas bearing the Rd8 mutation of Crb1 are characterized by the presence of intralesional bacteria. While normal CRB1 expression was enriched in the apical junctional complexes of retinal pigment epithelium and colonic enterocytes, Crb1 mutations dampened its expression at both sites. Consequent impairment of the outer blood retinal barrier and colonic intestinal epithelial barrier in Rd8 mice led to the translocation of intestinal bacteria from the lower gastrointestinal (GI) tract to the retina, resulting in secondary retinal degeneration. Either the depletion of bacteria systemically or the reintroduction of normal Crb1 expression colonically rescued Rd8-mutation-associated retinal degeneration without reversing the retinal barrier breach. Our data elucidate the pathogenesis of Crb1-mutation-associated retinal degenerations and suggest that antimicrobial agents have the potential to treat this devastating blinding disease.
作者机构：
[Dai, Hailu; He, Shoucheng] Yancheng Inst Technol, Sch Mat Sci & Engn, Yancheng 224051, Peoples R China.;[Bi, Lei] Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
通讯机构：
[Bi, L ] U;Univ South China, Sch Resource Environm & Safety Engn, Hengyang 421001, Peoples R China.
关键词：
Sb doping;Cathode;LaFeO3;PCFCs;SOFCs
摘要：
The La0.5Sr0.5FeO3-delta (LSF) material is modified with Sb doping to improve the performance of conventional LSF cathode for protonic ceramic fuel cells (PCFCs). The Sb cations can be inserted into the LSF lattice to develop a new composition La0.5Sr0.5Fe0.9Sb0.1O3-delta (LSFSb), which reduces the formation energy of oxygen vacancies and lowers the proton migration energy of the Sb-doped material when compared to the Sb-free LSF. Additional experimental findings reveal that Sb doping enhances the proton/oxygen transport and surface exchange kinetics. The fuel cell with the LSFSb cathode has a substantially higher peak power density of 933 mW cm-2 at 700 degrees C than the LSF cell, which only reaches 606 mW cm-2 under the same testing conditions. Using the LSFSb composite cathode further improved the fuel-cell performance, resulting in a promising fuel cell performance of 1388 mW cm-2, which is high for LSF-based PCFCs, thus demonstrating that LSFSb is a suitable cathode for PCFCs.
作者机构：
[Zeng, Huilan] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Ophthalmol, Hengyang 421001, Hunan, Peoples R China.;[Wadkins, David; Mayberry, Jordan E.; Kuehn, Markus H.; Chen, Nathan] Univ Iowa, Dept Ophthalmol & Visual Sci, Iowa City, IA 52242 USA.;[Wadkins, David; Mayberry, Jordan E.; Kuehn, Markus H.; Chen, Nathan] Iowa City VA Ctr Prevent & Treatment Visual Loss, Iowa City, IA 52246 USA.;[Summers, Daniel W.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA.
通讯机构：
[Kuehn, MH ] U;Univ Iowa, Dept Ophthalmol & Visual Sci, Iowa City, IA 52242 USA.;Iowa City VA Ctr Prevent & Treatment Visual Loss, Iowa City, IA 52246 USA.
摘要：
Glaucoma is one of the leading causes of irreversible blindness worldwide and vision loss in the disease results from the deterioration of retinal ganglion cells (RGC) and their axons. Metabolic dysfunction of RGC plays a significant role in the onset and progression of the disease in both human patients and rodent models, highlighting the need to better define the mechanisms regulating cellular energy metabolism in glaucoma. This study sought to determine if Sarm1, a gene involved in axonal degeneration and NAD+ metabolism, contributes to glaucomatous RGC loss in a mouse model with chronic elevated intraocular pressure (IOP). Our data demonstrate that after 16weeks of elevated IOP, Sarm1 knockout (KO) mice retain significantly more RGC than control animals. Sarm1 KO mice also performed significantly better when compared to control mice during optomotor testing, indicating that visual function is preserved in this group. Our findings also indicate that Sarm1 KO mice display mild ocular developmental abnormalities, including reduced optic nerve axon diameter and lower visual acuity than controls. Finally, we present data to indicate that SARM1 expression in the optic nerve is most prominently associated with oligodendrocytes. Taken together, these data suggest that attenuating Sarm1 activity through gene therapy, pharmacologic inhibition, or NAD+ supplementation, may be a novel therapeutic approach for patients with glaucoma.
期刊：
Scandinavian Journal of Immunology,2024年99(3):e13357- ISSN：0300-9475
通讯作者：
Lei, AH;Zhang, HJ
作者机构：
[Long, WeiXiang; Lei, AH; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Inst Pathogen Biol, Sch Basic Med Sci, Hengyang Med Sch, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.;[Long, WeiXiang; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Hunan Prov Key Lab Special Pathogens Prevent & Con, Hengyang, Peoples R China.;[Long, WeiXiang; Lei, Aihua; Dai, Zhongling; Liu, Duo; Gong, Zhande; Wang, Cui] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dru, Hengyang, Peoples R China.;[Zhang, Haijun] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Cardiol, 69 Chuanshan Ave, Hengyang 421000, Hunan, Peoples R China.
通讯机构：
[Lei, AH ; Zhang, HJ ] U;Univ South China, Inst Pathogen Biol, Sch Basic Med Sci, Hengyang Med Sch, 28 Changsheng Western Rd, Hengyang 421001, Hunan, Peoples R China.;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Cardiol, 69 Chuanshan Ave, Hengyang 421000, Hunan, Peoples R China.
关键词：
allergic airway inflammation;hormones;ILC2s
摘要：
Group 2 innate lymphoid cells (ILC2s) are a type of innate immune cells that produce a large amount of IL-5 and IL-13 and two cytokines that are crucial for various processes such as allergic airway inflammation, tissue repair and tissue homeostasis. It is known that damaged epithelial-derived alarmins, such as IL-33, IL-25 and thymic stromal lymphopoietin (TSLP), are the predominant ILC2 activators that mediate the production of type 2 cytokines. In recent years, abundant studies have found that many factors can regulate ILC2 development and function. Hormones synthesized by the body's endocrine glands or cells play an important role in immune response. Notably, ILC2s express hormone receptors and their proliferation and function can be modulated by multiple hormones during allergic airway inflammation. Here, we summarize the effects of multiple hormones on ILC2-driven allergic airway inflammation and discuss the underlying mechanisms and potential therapeutic significance.
摘要：
BACKGROUND: Neuronal ferroptosis plays a critical role in the pathogenesis of cognitive deficits. The present study explored whether artemisinin protected type 2 diabetes mellitus (T2DM) mice from cognitive impairments by attenuating neuronal ferroptosis in the hippocampal CA1 region. METHODS: STZ-induced T2DM mice were treated with artemisinin (40 mg/kg, i.p.), or cotreated with artemisinin and Nrf2 inhibitor MEL385 or ferroptosis inducer erastin for 4 weeks. Cognitive performance was determined by the Morris water maze and Y maze tests. Hippocampal ROS, MDA, GSH, and Fe(2+) contents were detected by assay kits. Nrf2, p-Nrf2, HO-1, and GPX4 proteins in hippocampal CA1 were assessed by Western blotting. Hippocampal neuron injury and mitochondrial morphology were observed using H&E staining and a transmission electron microscope, respectively. RESULTS: Artemisinin reversed diabetic cognitive impairments, decreased the concentrations of ROS, MDA and Fe(2+), and increased the levels of p-Nr2, HO-1, GPX4 and GSH. Moreover, artemisinin alleviated neuronal loss and ferroptosis in the hippocampal CA1 region. However, these neuroprotective effects of artemisinin were abolished by Nrf2 inhibitor ML385 and ferroptosis inducer erastin. CONCLUSION: Artemisinin effectively ameliorates neuropathological changes and learning and memory decline in T2DM mice; the underlying mechanism involves the activation of Nrf2 to inhibit neuronal ferroptosis in the hippocampus.
