期刊论文

DADS Inhibit EMT Through Down-Regulation of β-catenin in Human Gastric Cancer MGC803 Cells

中文

中南医学科学杂志

2095-1116

2015

5

487-492,514

10.15972/j.cnki.43-1509/r.2015.05.002

81102854,31100935,81374013:国家自然科学基金 B2015-182:湖南省卫生计生委科研项目

本校为第一机构

目的研究二烯丙基二硫（DADS）抑制人胃癌MGC803细胞EMT与形态学变化。方法应用相差显微镜与透射电镜观察30 mg·L-1DADS处理后的MGC803细胞形态学与超微结构的变化。RT-PCR与Western blot检测β-catenin和EMT标记E-cadherin与Vimentin表达。结果 30 mg·L-1DADS处理MGC803细胞24 h后,相差显微镜显示,与未处理比较,细胞圆形、椭圆形,核浆比值降低,巢状分布,均未见伪足。透射电镜显示,细胞表面微绒毛数目减少,细胞核变规整,核浆比值下降,异型性降低,细胞与细胞之间出现连接结构,细胞器增多。RT-PCR与Western blot表明,DADS可呈时间依赖性分别下调MGC803细胞β-catenin和Vimentin mRNA与蛋白和上调...

Objective To investigate the effect of EMT and morphology change in human gastric cancer MGC803 cells induced by diallyl disulfide (DADS). Methods The phase contrast microscope and transmission electron micro-scope were employed to confirm the DADS-induced morphology change. The expression ofβ-catenin,E-cadherin and Vimen-tin was detected by RT-PCR and Western blot in MGC803 cells after treated by 30 mg·L-1 DADS. Results The phase contrast microscope show that MGC803 cells after 24 h treated by 30 mg·L-1 DADS were round or ellipse,enlarged c...