期刊论文

中文

中南医学科学杂志

2095-1116

2014

3

217-221

10.15972/j.cnki.43-1509/r.2014.03.009

国家自然科学基金资助项目（81370378）；

本校为第一机构

目的探讨氧化型低密度脂蛋白( Ox-LDL)对PMA诱导的THP-1巨噬细胞自噬的影响。方法PMA诱导THP-1细胞24 h,使其分化为巨噬细胞,再分别用0、10、20、40或80 mg/L的Ox-LDL处理24 h,逆转录聚合酶链反应( RT-PCR)和免疫印迹技术( Western blotting)分别检测Beclin-1以及LC3的mRNA及蛋白表达；细胞免疫荧光检测LC3在细胞内的含量；MDC染色检测自噬囊泡的形成。结果随着Ox-LDL浓度的增加,THP-1源性巨噬细胞Beclin-1 mRNA及蛋白表达显著降低(P0.05),但蛋白水平LC3II/LC3I显著降低(P

Objective To explore the effect of oxidized low density lipoprotein （ Ox-LDL） on autophagy in THP-1-derived macrophages induced by phorbol-12-myristate-13-acetate （ PMA ） . Methods After treatment with PMA for 24 h,THP-1-derived macrophages were treated with 0,10,20,40 or 80 mg/L Ox-LDL for 24 h. Reverse transcription poly-merase chain reaction （ RT-PCR） and Western blotting were used to detect Beclin 1 and LC3 mRNA and protein expres-sion. Cell immunofluorescence was used to detect intracellular LC3II protein concentration. The autofluor...