Discrimination of A1555G and C1494T point mutations in the mitochondrial 12S rRNA Gene by On/Off switch

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Guo, Zi-Fen;Guo, Wu-Shuang;Xiao, Li;Gao, Guo-Qiang;Lan, Fen;...

通讯作者：

Li, Kai

作者机构：

[Guo, Zi-Fen; Lan, Fen; Gao, Guo-Qiang; Guo, Wu-Shuang; Liao, Duan-Fang] Univ S China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.

[Li, Kai] Soochow Univ, Coll Med, Dept Pharmacol, Suzhou 215123, Jiangsu, Peoples R China.

[Lu, Xue-Guan; Xiao, Li] Soochow Univ, Affiliated Hosp 2, Mol Med Ctr, Suzhou 215004, Jiangsu, Peoples R China.

[Liao, Duan-Fang] Hunan Univ Chinese Med, Sch Pharm, Dept Tradit Chinese Diognot, Changsha 410208, Hunan, Peoples R China.

通讯机构：

[Li, Kai] S

Soochow Univ, Coll Med, Dept Pharmacol, Suzhou 215123, Jiangsu, Peoples R China.

语种：

英文

关键词：

Aminoglycoside antibiotics;Exo + polymerase;Mitochondrial 12S rRNA;Phosphorothioate modification

期刊：

Applied Biochemistry and Biotechnology

ISSN：

0273-2289

年：

2012

卷：

166

期：

页码：

234-242

DOI：

10.1007/s12010-011-9419-4

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81102516]; National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2008AA02Z436]; Hunan Provincial Science and Technology Department in China [2011SK3087]

机构署名：

本校为第一机构

院系归属：

药学与生物科学学院

摘要：

The objective of this study was to apply the "on/off" switch consisting of 3′ phosphorothioate-modified allele specific primers and exo + polymerase in single base discrimination of A1555G and C1494T mutations in the highly conserved sites of the mitochondrial 12S rRNA. The two point mutations are the hotspot mutations associated with either aminoglycoside antibiotics induced deafness or inherited nonsyndromic hearing loss. The PCR products of mitochondrial DNA (mtDNA) 12S rRNA gene were inserted into the pMD19-T vector for transformation into...

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Discrimination of A1555G and C1494T point mutations in the mitochondrial 12S rRNA Gene by On/Off switch

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