期刊论文

中文

辐射防护

1000-8187

2009

29

4

225-231

国家科技部重大专项课题（课题号：2008ZXJ09001-008） 国家自然科学基金资助课题（课题号：3067634）.

本校为第一机构

采用单细胞凝胶电泳、微核实验、AnnexinV标记结合流式细胞术、双荧光染色结合荧光显微镜观察等方法,分别检测不同浓度的VND3207对~(60)Coγ射线照射人淋巴母细胞AHH-1基因组损伤和凋亡的防护作用.中性单细胞凝胶电泳结果显示,5-40μmol/L VND3207能显著减轻2 Gy ~(60)Coγ射线照射AHH-1细胞的即刻DNA双链断裂损伤,其表现为与未加药组相比,VND3207保护组细胞的DNA双链断裂损伤(彗星尾矩)显著减小(p

To determine the protective effect of vanillin derivative VND3207 on the genome damage and apoptosis of human lymphoblastoid AHH-1 cells induced by γ-ray irradiation, the techniques of single-cell gel electrophoresis, micronueleus test, Annexin V-FACS assay, and the double-fluorescein staining and fluorescent microscope observation were used. Neutral single-cell gel electrophoresis showed that the initial DNA double-strand breaks caused by 2 Gy ^60Co γ-ray was significantly decreased by VND3207 in the range of 5-40 μ mol/L. This significant ...