期刊论文

Liu, Lu-Shan

英文

Molecular and Cellular Biochemistry

0300-8177

2012

359

1-2

347-358

10.1007/s11010-011-1028-6

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30700325]; Educational Department of Hunan Province [10A105]; Science and Technology Department of Hunan Province [2009TP4057-2, 2010TP4008-2]; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

本校为第一且通讯机构

This paper investigated the effects of ox-LDL on PCSK9, and the molecular mechanisms of PCSK9 siRNA-inhibited apoptosis induced by ox-LDL in human umbilical vein endothelial cells (HUVECs), to clarify the role of PCSK9 in atherosclerogenesis. HUVECs were incubated with ox-LDL for 24 h. The apoptosis was observed by Hoechst 33258 staining. The expression of PCSK9, LOX-1 mRNAs and proteins was detected by RT-PCR, western blot, respectively. The PCSK9 siRNAs labeled with fluorescence were transfected into HUVECs by Lipofectamine 2000. After transf...