期刊论文

中文

中国人兽共患病学报

1002-2694

2005

21

7

561-565

10.3969/j.issn.1002-2694.2005.07.005

本校为其他机构

目的研究H. pylori作用后肝细胞系HepG2蛋白质表达谱的改变,通过对差异蛋白质点的鉴定与分析,以探讨H. pylori对肝细胞的病理作用.方法采用体外肝细胞和H. pylori共培养,应用双向凝胶电泳分离H. pylori处理前后细胞总蛋白,筛选出差异表达的蛋白质点,并进行质谱分析鉴定.结果鉴定出7种表达上调的蛋白质点,包括整合素β-1、蛋白激酶C-α、LIM同源盒蛋白Lhx1、真核翻译起始因子2-β亚单位、PINCH蛋白、MAPK激酶3、Ras 相关蛋白Rab-37等.这些蛋白质参与了基因表达调控、细胞免疫、细胞生长、信号传导等众多事件.结论 H. pylori可能通过介导这些蛋白质的上调而发挥对HepG2细胞的病理作用.这种蛋白质组...

To explore the pathological effect of H. pylori on human hepatic cells, this study was conducted to investigate the change of the protein expression of HepG2 caused by H. pylori treatment, and to identify differential expression proteins. H. pylori was co-cultured with HepG2 for 6h, two-dimensional gel eletrophoresis (2-DE) was used to gain the protein pattern of HepG2 cells before and after treated with H. pylori. The differential expression proteins were identified by MALDI-TOF-MS. Seven proteins which were up-regulated in H. pylori treated HepG2 were identified. These proteins included inte...