期刊论文

Lin, Ying Wu

英文

中国化学快报：英文版

1001-8417

2012

23

6

741-744

10.1016/j.cclet.2012.03.025

It is a pleasure to acknowledge Professor S.G. Sligar and Dr. Yi Lu at University of Illinois at Urbana-Champaign, for their kind gift of Mb gene. NAMD and VMD were developed by the Theoretical Biophysics Group in the Beckman Institute for Advanced Science and Technology at the University of Illinois at Urbana-Champaign. This work was supported by the National Natural Science Foundation of China, NSFC (No. 21101091 ), Hunan Provincial Natural Science Foundation (No. 11JJ4017 ), Hunan Provincial Education Foundation (No. 11B105 ), and the initial foundation for oversea scholar return to University of South China (No. 2011XQD16 ).

本校为第一且通讯机构

By retaining the native distal His64 in sperm whale myoglobin (Mb), a second distal histidine was engineered in Mb by mutating Leu29 to His29. The resultant mutant of L29H Mb exhibits an unusual enhanced peroxidase activity with a positive cooperativity in comparison to that of wild type Mb. The new enzyme with two cooperative distal histidines has not been found in native peroxidase, which emphasizes a creation of...

By retaining the native distal His64 in sperm whale myoglobin（Mb）,a second distal histidine was engineered in Mb by mutating Leu29 to His29.The resultant mutant of L29H Mb exhibits an unusual enhanced peroxidase activity with a positive cooperativity in comparison to that of wild type Mb.The new enzyme with two cooperative distal histidines has not been found in native peroxidase, which emphasizes...