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Double-receptor sandwich supramolecule sensing method for the determination of ATP based on uranyl-salophen complex and aptamer

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成果类型:
期刊论文
作者:
Zhao, Minmin;Liao, Lifu*;Wu, Minlong;Lin, Yingwu;Xiao, Xilin;...
通讯作者:
Liao, Lifu
作者机构:
[Wu, Minlong; Zhao, Minmin; Xiao, Xilin; Liao, Lifu; Nie, Changming; Lin, Yingwu] Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Liao, Lifu] U
Univ S China, Coll Chem & Chem Engn, Hengyang 421001, Hunan, Peoples R China.
语种:
英文
关键词:
Aptamer;ATP;Double-receptor;Sandwich supramolecule;Uranyl-salophen complex
期刊:
Biosensors and Bioelectronics
ISSN:
0956-5663
年:
2012
卷:
34
期:
1
页码:
106-111
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [10975069, 20877038, 21101091]; Hunan Provincial Education DepartmentHunan Provincial Education Department [09C851]
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
摘要:
In this study, we report a double-receptor sandwich supramolecule sensing method for the determination of adenosine triphosphate (ATP). One receptor is a uranyl-salophen complex which can bind the triphosphate group in ATP selectively, and another is an anti-adenosine aptamer which is a single-stranded oligonucleotide and can recognize the adenosine group in ATP specifically. The uranyl-salophen complex was immobilized on the surface of amino-silica gel particles and used as the solid phase receptor of ATP. The anti-adenosine aptamer was labele...

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