期刊论文

Jiang, J.

中文

细胞与分子免疫学杂志

1007-8738

2013

29

8

785-788

10.13423/j.cnki.cjcmi.008208

湖南省教育厅重点项目（11A103） 湖南省大学生研究性学习和创新性实验计划项目（湘教通[2011]272号）

本校为第一机构

目的 研究NLRP3炎症小体信号通路相关分子在幽门螺杆菌（H.pylori）感染C57BL/6小鼠胃组织和血清中的表达情况, 初步探讨 NLRP3炎症小体信号通路在H.pylori致病中的作用。方法 将C57BL/6小鼠分为H.pylori感染组和PBS对照组, 于感染后不同时间点处死小鼠, RT-PCR检测小鼠胃组织中NLRP3炎症小体和下游信号通路分子mRNA表达, Western blot法检测小鼠胃组织中caspase-1蛋白表达, ELISA测定小鼠血清中IL-1β、IL-18和IL-33含量。结果 与对照组相比, H.pylori感染组小鼠胃黏膜均呈慢性炎症改变, 且随时间延长, 炎症程度逐渐加重。NLRP3炎症小体信号通路相关分子mRNA及caspase-1蛋白表达水平显著升高, ...

OBJECTIVE: To study the expressions of NLRP3 inflammasome and its downstream molecules in the gastric tissues and sera of Helicobacter pylori (H.pylori)-infected C57BL/6 mice, and to preliminary explore the role of NLRP3 inflammasome signalling pathway in the pathogenesis of H.pylori infection. METHODS: C57BL/6 mice were randomly divided into H.pylori infection group and PBS control group, and mice were sacrificed at different time points after H.pylori infection. The mRNA expressions of NLRP3 inflammasome and downstream signalling pathway related molecules in mouse gastric tissues were detect...