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Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China

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WOS被引频次:109
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成果类型:
期刊论文
作者:
Weiyi Fang;Xin Li;Qingping Jiang;Zhen Liu;Huiling Yang;Shuang Wang;Siming Xie;Qiuzhen Liu;Tengfei Liu;Jing Huang;Weibing Xie;Zuguo Li;Yingdong Zhao;Ena Wang;Francesco M Marincola;Kaitai Yao
通讯作者:
Marincola, Francesco M.
作者机构:
[Weiyi Fang; Xin Li; Zhen Liu; Huiling Yang; Siming Xie; Qiuzhen Liu; Tengfei Liu; Jing Huang; Weibing Xie; Zuguo Li; Yingdong Zhao] Cancer Research Institute of Southern Medical University
[Qingping Jiang; Francesco M Marincola; Kaitai Yao] Warren G. Magnuson Clinical Center
[Shuang Wang] The first affiliated hospital of Nanhua University
[Ena Wang] National Cancer Institute
通讯机构:
[Marincola, Francesco M.] NIH, Warren G Magnuson Clin Ctr, Infect Dis & Immunogenet Sect, Dept Transfus Med, Bethesda, MD 20892 USA.
Warren G. Magnuson Clinical Center
语种:
英文
期刊:
Journal of Translational Medicine
ISSN:
1479-5876
年:
2008
卷:
6
期:
1
页码:
32-
文献类别:
WOS:Article
所属学科:
ESI学科类别:临床医学;WOS学科类别:Medicine, Research & Experimental
入藏号:
WOS:000257608300001;PMID:18570662
机构署名:
本校为其他机构
摘要:
BACKGROUND: The pathogenesis of nasopharyngeal carcinoma (NPC) is a complicated process involving genetic predisposition, Epstein-Bar Virus infection, and genetic alterations. Although some oncogenes and tumor suppressor genes have been previously reported in NPC, a complete understanding of the pathogenesis of NPC in the context of global gene expression, transcriptional pathways and biomarker assessment remains to be elucidated. METHODS: Total RNA from 32 pathologically-confirmed cases of poorly-differentiated NPC was divided into pools inclusive of four consecutive specimens and each pool (T1 to T8) was co-hybridized with pooled RNA from 24 normal non-cancerous nasopharyngeal tissues (NP) to a human 8K cDNA array platform. The reliability of microarray data was validated for selected genes by semi-quantitative RT-PCR and immunohistochemistry. RESULTS: Stringent statistical filtering parameters identified 435 genes to be up-regulated and 257 genes to be down-regulated in NPC compared to NP. Seven up-regulated genes including CYC1, MIF, LAMB3, TUBB2, UBE2C and TRAP1 had been previously proposed as candidate common cancer biomarkers based on a previous extensive comparison among various cancers and normal tissues which did not, however, include NPC or NP. In addition, nine known oncogenes and tumor suppressor genes, MIF, BIRC5, PTTG1, ATM, FOXO1A, TGFBR2, PRKAR1A, KLF5 and PDCD4 were identified through the microarray literature-based annotation search engine MILANO, suggesting these genes may be specifically involved in the promotion of the malignant conversion of nasopharyngeal epithelium. Finally, we found that these differentially expressed genes were involved in apoptosis, MAPK, VEGF and B cell receptor signaling pathways and other functions associated with cell growth, signal transduction and immune system activation. CONCLUSION: This study identified potential candidate biomarkers, oncogenes/tumor suppressor genes involved in several pathways relevant to the oncogenesis of NPC. This information may facilitate the determination of diagnostic and therapeutic targets for NPC as well as provide insights about the molecular pathogenesis of NPC.
参考文献:
Sriuranpong V, Mutirangura A, Gillespie JW, Patel V, Amornphimoltham P, Molinolo AA, Kerekhanjanarong V, Supanakorn S, Supiyaphun P, Rangdaeng S, Voravud N, Gutkind JS: Global gene expression profile of nasopharyngeal carcinoma by laser capture microdissection and complementary DNA microarrays. Clin Cancer Res. 2004, 10 (15): 4944-4958. 10.1158/1078-0432.CCR-03-0757.
Li RP, Shao JY, Deng L, Zeng MS, Song LB, Li MZ, Wu QL: [Identification of differentially expressed genes in primary cultured nasopharyngeal carcinoma cells by cDNA microarray]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2007, 27 (8): 1156-1160.
Zhou L, Jiang W, Ren C, Yin Z, Feng X, Liu W, Tao Q, Yao K: Frequent hypermethylation of RASSF1A and TSLC1, and high viral load of Epstein-Barr Virus DNA in nasopharyngeal carcinoma and matched tumor-adjacent tissues. Neoplasia. 2005, 7 (9): 809-815. 10.1593/neo.05217.
Qiu GH, Tan LK, Loh KS, Lim CY, Srivastava G, Tsai ST, Tsao SW, Tao Q: The candidate tumor suppressor gene BLU, located at the commonly deleted region 3p21.3, is an E2F-regulated, stress-responsive gene and inactivated by both epigenetic and genetic mechanisms in nasopharyngeal carcinoma. Oncogene. 2004, 23 (27): 4793-4806. 10.1038/sj.onc.1207632.
Yan W, Song L, Wei W, Li A, Liu J, Fang Y: Chromosomal abnormalities associated with neck nodal metastasis in nasopharyngeal carcinoma. Tumour Biol. 2005, 26 (6): 306-312. 10.1159/000089289.

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