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阴离子交换色谱-电感耦合等离子体质谱法测定长期汞暴露人群硒干预后血清中的小分子硒形态

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成果类型:
期刊论文
作者:
Hu Liang;Dong Ze-Qin;Huang Xiao-Han;Li Yu-Feng*;Li Bai;...
通讯作者:
Li Yu-Feng
作者机构:
[Li Bai; Gao Yu-Xi; Hu Liang; Chen Chun-Ying; Huang Xiao-Han; Li Yu-Feng] Chinese Acad Sci, Multidiscipline Initiat Ctr, Inst High Phys, Beijing 100049, Peoples R China.
[Li Bai; Hu Liang; Huang Xiao-Han; Wang Guo-Ping] Univ S China, Sch Chem & Chem, Hengyang 421001, Peoples R China.
[Dong Ze-Qin; Qu Li-Ya] Guizhou Inst Environm Sci & Designing, Guiyang 550002, Peoples R China.
[Chen Chun-Ying] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China.
通讯机构:
[Li Yu-Feng] C
Chinese Acad Sci, Multidiscipline Initiat Ctr, Inst High Phys, Beijing 100049, Peoples R China.
语种:
中文
关键词:
阴离子交换色谱;电感耦合等离子体质谱;硒;形态
关键词(英文):
Anion exchange chromatography;Inductively coupled plasma mass spectrometry;Selenium species;Serum
期刊:
分析化学
ISSN:
0253-3820
年:
2011
卷:
39
期:
4
页码:
466-470
基金类别:
Received 7 August 2010; accepted 25 September 2010 * Corresponding author. Email: liyf@ihep.ac.cn This work was supported by the Knowledge Innovation Programme of the Chinese Academy of Sciences (No. KJCX3.SYW.N3), the International Atomic Energy Agency of China (No. CPR-15818), and the EU sixth Framework Programme of China (No. FOOD-CT-2006-016253). Copyright © 2011, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Published by Elsevier Limited. All rights reserved. DOI: 10.1016/S1872-2040(10)60432-X
机构署名:
本校为其他机构
院系归属:
化学化工学院
摘要:
建立了阴离子交换高效液相色谱-电感耦合等离子体质谱联用测定不同形态硒的在线分离检测方法,并将其应用于长期汞暴露人群血清中小分子硒的形态研究.流动相A为0.5 mmol/L柠檬酸铵-2%(V/V)甲醇(pH 3.7),流动相B为100 mmol/L柠檬酸铵-2%(V/V)甲醇(pH 8.0);采用93%A-7%B时,硒代半胱氨酸(SeCys)、硒代蛋氨酸(SeMet)、Se(Ⅳ)和Se(Ⅵ)的保留时间分别为2.48,3.28,5.19和7.02 min.采用基体匹配标准外标法定量,加标回收率为93%~117%;相对标准偏差小于9%;SeCys,AeMet,Se(Ⅳ)和 Se(Ⅵ)的检出限分别为0.34,0.67,1.38和0.63μg/L(S/N=3).本方法已成功应用于研究长期汞暴露地区硒干预人群中血清中硒的化学形态...
摘要(英文):
A hyphenated technique was optimized to simultaneously determine different selenium species, including Se(IV), Se(VI), selenocysteine (SeCys), and selenomethionine (SeMet), by anion exchange chromatography (AEC)-inductively coupled plasma mass spectrometry (ICP-MS). Ammonium citrate and methanol were used as mobile phase. Mobile phase A is composed of 0.5 mM ammonium citrate + 2% (V/V) methanol, and mobile phase B is 100 mM ammonium citrate + 2% (V/V) methanol. When 93% A + 7% B were used, the four Se species can be separated in 7.5 min. The de...

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