期刊论文

Chen, Linxi

英文

生物化学与生物物理学报

1672-9145

2010

42

6

396-402

10.1093/abbs/gmq035

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [3090577]; Hengyang Municipal Bureau of Science and Technology [2009KJ14]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars

本校为通讯机构

Vascular smooth muscle cells (VSMCs) were prepared from thoracic aortas of male Sprague-Dawley rats by the explant method to observe VSMC proliferation via phosphoinositide 3 kinase (PI3K)/Akt signaling transduction pathway induced by apelin-13. Expression of PI3K, phospho-PI3K, phospho-Akt, ERK1/2, phospho-ERK1/2 and cyclin D1 was detected by western blot analysis. Results showed that apelin-13 promoted the expression of phospho-PI3K and phospho-Akt in dose- and time-dependent manner. PI3K inhibitor LY294002 significantly decreased the express...

脉管的光滑的肌肉房间(VSMC ) 被将生物的活组织移植于培养基中培养方法从男 SpragueDawley 老鼠的胸的主动脉准备经由 3 kinase (PI3K )/Akt 发信号 transduction 小径由 apelin-13 导致了的 phosphoinositide 观察 VSMC 增长。PI3K， phospho-PI3K， phospho-Akt， ERK1/2， phospho-ERK1/2 和 cyclin D1 的表示被西方的污点分析检测。结果证明 apelin-13 在剂量依赖者和时间依赖者举止支持了 phospho-PI3K 和 phospho-Akt 的表示。PI3K 禁止者 LY294002 显著地减少了 phospho-PI3K， phospho-Akt， phospho-ERK1/2，和 cyclin D1 的表示由 apelin-13 导致了。Akt 禁止者 1701-1 显著地减少了...