期刊论文

中文

中国现代医药杂志

1672-9463

2010

12

5

1-4

10.3969/j.issn.1672-9463.2010.05.001

湖南省高校创新平台开放基金项目（课题号:09K074）； 南华大学博士科研启动基金资助项目（课题号:2007XQD26）；

本校为第一机构

目的 探讨二烯丙基二硫(diallyl disulfide,DADS)对人胶质瘤U251细胞增殖及维甲酸相关孤核受体α(RORα)蛋白表达的影响.方法 采用MTT比色实验、群体倍增时间分析DADS对人胶质瘤U251细胞的生长抑制效应;Western blot技术分析RORα蛋白在DADS处理U251细胞前后的表达改变.结果 DADS浓度从15、22.5、30、37.5到45mg/L处理U251细胞,其吸光值比对照组低,而且随浓度增加逐渐降低(P

Objective To investigate the arrest effect of diallyl disulfide （DADS） in the proliferation and retinoic acid-related orphan receptor-alpha （ROR alpha） expression of human glioma cell line U251. Methods MTT assay,cell average doubling time analysis were employed to confirm the DADS-induced cell growth inhibition in cell line U251; Western Blot was used to observed expression of ROR alpha protein in U251 cells by 30mg/L DADS. Results Apparently growth inhibition in U251 cell line treated with DADS could be seen and exhibited a dose-dependent...