期刊论文

Overexpression of Chk1/2 gene affectsG_2/M arrest in MGC803 cells induced by diallyl disulfide

中文

中国药理学通报

1001-1978

2016

32

2

199-204

10.3969/j.issn.1001-1978.2016.02.011

国家自然科学基金资助项目（No81102854,81374013） 湖南省卫生厅科研课题（NoB2015-182）

本校为第一机构

目的在建立Chk1/2基因高表达人胃癌MGC803细胞基础上,探讨DADS对Chk1/2高表达MGC803细胞G_2/M期的作用。方法分别采用集落形成实验、流式细胞术、RT-PCR、Western blot等方法,检测DADS对Chk1/2高表达MGC803细胞增殖、细胞周期分布、Chk1与Chk2 mRNA与蛋白、p-Chk1与p-Chk2及CDC25C与cyclinB1的表达。结果软琼脂集落形成实验显示,30 mg·L~(-1) DADS作用Chk1与Chk2高表达MGC803细胞组集落形成率均明显低于对照组与空载体组(P

Aim To investigate the effects of diallyl di-sulfide( DADS) on G2/M arrest in Chk1/MGC803 and Chk2/MGC803 cells so as to establish stable human gastric cancer MGC803 cells with overexpression of Chk1/2 gene. Methods The colony formation, flow cytometry, RT-PCR and Western blot were used to de-tect the proliferation, cell cycle, and expression of Chk1/2 mRNA and protein, p-Chk1/2, CDC25C and cyclinB1, respectively. Results The colony formation showed that the colony forming efficiency in Chk1/MGC803 and Chk2/MGC803 cells treated by 30 mg· ...