期刊论文

中文

中国动脉硬化杂志

1007-3949

2006

14

10

841-844

10.3969/j.issn.1007-3949.2006.10.003

国家自然科学基金（30400169,30571746,30571846）； 国家973重点项目（G2000056900）资助；

本校为其他机构

目的 观察Kruppel样因子4过表达对热休克蛋白25表达的影响。方法 运用稳定转染pcDNA3.1/myc-His(一)和Kruppel样因子4-pcDNA3.1/myc-His(一)两种细胞株，采用逆转录聚合酶链反应观察Kruppel样因子4在生理状态和热休克反应时对热休克蛋白25mRNA表达的影响；采用Western blot观察Kruppel样因子4在生理状态和热体克反应时对热体克蛋白25蛋白表达的影响。结果 在正常状态下，两种细胞热休克蛋白25mRNA及蚕白表达水平均较低。生理状态下，热休克恢复1h及3h Kruppel样因子4过表达细胞株热休克蛋白25mRNA表达水平明显高于转空载体细胞；至热体克恢复6h后两组细胞热休克蛋白25mRNA表达无明显差异。在生理...

Aim To investigate the effects of Kruppel-like factor 4（KLF4）. overexpression on the expression of heat shock protein25 （HSP25）. Methods Two stable cell lines were made by transforming pcDNA3, 1/Myc-His （-） or pcDNA3. 1/Myc-His-KLF4 into C2 C12 cell. Reverse transcription polyrnerase chain reaefion（RT-PCR） and Western Blot were employed to study the influence of KLF4 on the expression of HSP25 mRNA and protein respectively under normal physiological and heat shock conditions. Results Under normal physiological condition, the expression ...