期刊论文

Li Lan-Fang

英文

生物化学与生物物理进展

1000-3282

2011

38

12

1162-1170

10.3724/SP.J.1206.2011.00335

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30901577, 30770868]; National Basic Research Program of ChinaNational Basic Research Program of China [2006CB503808]; USC [2008XQD01]; Hengyang Municipal Bureau of Science and Technology [2009KJ14]; Ministry of Education of Returned Overseas Students [20091590]; Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province

本校为第一且通讯机构

Previously we reported that G protein-coupled receptor APJ endogenous ligand apelin-13 induced adhesion of monocytes to human umbilical vein endothelial cells (HUVECs).Now we investigated whether phosphatidylinositol 3-kinase (PI3K) signaling pathway mediated monocytes (MCs) adhesion to HUVECs induced by apelin-13. Human umbilical vein endothelial cell line ECV304 was cultured in DMEM medium and the monocyte cell line THP-1 was cultured in 1640 medium. Myeloperoxidase(MPO) assay was used to identify effects of monocytes adhesion to HUVECs. Expression of vascular cell adhesion molecule (VCAM)-1...

本室以前已经报道G蛋白偶联受体APJ的内源性配体多肽apelin-13促进单核细胞-血管内皮细胞黏附,本文研究PI3K信号途径是否参与apelin-13促进单核细胞-血管内皮细胞黏附,探讨apelin/APJ系统的细胞信号转导机制.MPO方法检测细胞黏附;Western blot方法检测PI3K、VCAM-1的表达.Western blot方法结果显示,apelin-13（0、0.5、1、2、4μmol/L）浓度依赖性刺激血管内皮细胞PI3K磷酸化,以1μmol/L最为明显;1μmol/L apelin-13时间依赖性促进血管内皮细胞PI3K磷酸化,在30 min增加最为显著;PI3K抑制剂LY294002明显抑制apelin-13诱导的VCAM-1表达和单核细胞-血管内皮细胞黏附.上述结果表明,PI3K信号途径介导...