期刊论文、会议论文

Liang Xiao-Qiu

中文

生物化学与生物物理进展

1000-3282

2010

37

10

1138-1143

中国病理生理学会第十三届肿瘤、第十四届免疫专业委员会学术会议

2012-06-14

中国河南郑州

10.3724/SP.J.1206.2010.00168

Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [06JJ2048]; Hengyang Science and Technology Bureau of Hunan Province [2008KJ008]

本校为第一且通讯机构

为了探讨候选肝癌抑癌蛋白PIG11(p53-induced gene 11,PIG1 1)诱导细胞凋亡的机制,首次在HepG2细胞株中鉴定了1 1个PIG1 1结合蛋白,热休克蛋白60(heat shock protein 60,Hsp60)为其中之一。采用免疫共沉淀联合Western blot技术对Hsp60进行了验证。用Western blot检测其蛋白质表达,结果显示:pLXSN-PIG11

In order to explore the mechanisms of candidate liver tumor suppressor protein PIG11 induced apoptosis, eleven PIG11 binding proteins were first time identified in human hepatoma HepG2 cells, which heat shock protein 60 （Hsp60） is one of them. It has been confirmed by co-immunoprecipitation combined with Western blot analysis. Furthermore, using Western blot analysis, the protein expression of lisp60 is down-regulated in pLXSN-PIGll-HepG2 cells group than that of pLXSN-HepG2 and HepG2 groups （n =3, P 〈 0.01）, and over-expression of PIG11 i...