作者:
He, Min;Jiang, Ziyu;Tan, Wenfu;Li, Zhengmao;Peng, Bin
期刊:
Journal of Orthopaedic Surgery and Research,2023年18(1):1-5 ISSN:1749-799X
通讯作者:
Bin Peng
作者机构:
[He, Min; Jiang, Ziyu; Tan, Wenfu; Li, Zhengmao] Department of Traumatic Orthopedics, Hengyang Medical School, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, 421001, China;[Peng, Bin] Department of Traumatic Orthopedics, Hengyang Medical School, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, 421001, China. 2006pengbin@163.com
通讯机构:
[Bin Peng] D;Department of Traumatic Orthopedics, Hengyang Medical School, The Second Affiliated Hospital, University of South China, Hengyang, China
关键词:
Tibia;Retrograde intramedullary nail;Point of entry;Entry direction;Surgery
摘要:
To determine the ideal entry point and direction of retrograde intramedullary nailing of the tibia. The imaging data of patients with distal tibial fractures from June 2020 to December 2021 in our hospital were collected, and computer-aided design was performed. The relevant data were imported into the software for processing, so as to obtain a distal tibial fracture model and simulate the retrograde intramedullary nail placement in the tibia. The entry points and angles at which the intramedullary nail could be inserted successfully and the fracture could be maintained in good alignment were overlapped and counted to obtain the safe entry range and angle. The center of this safe range is the ideal entry point for retrograde intramedullary nailing of the tibia, and the mean value of the angle is the ideal direction of entry. The ideal entry point of the retrograde intramedullary nailing was located at the midpoint of the medial malleolus in the C-arm fluoroscopic anteroposterior (AP) and lateral view. The ideal nail entry direction was located at the anatomic axis of the medial malleolus in the AP position and at the anatomic axis of the distal tibial metaphysis in the lateral position. The ideal point and direction of nail insertion for retrograde tibial intramedullary nailing is a "double midpoint, double axis" approach.
摘要:
Ferroptosis is a novel type of cell death associated with iron accumulation and excessive lipid peroxidation. Elucidating the underlying molecular mechanisms of ferroptosis is intensively related to the development and treatment of multiple diseases, including musculoskeletal disorders. Moreover, in vitro and in vivo studies have shown the importance of oxidative stress in musculoskeletal conditions such as osteoporosis, osteoarthritis, rheumatoid arthritis, and osteosarcoma. Ferroptosis-derived clinical management of musculoskeletal diseases offers tremendous and attractive opportunities. Notably, ferroptosis agonists have been proven to enhance the sensitivity of osteosarcoma cells to conventional therapeutic strategies. In this review, we have mainly focused on the implications of ferroptosis regulation in the pathophysiology and therapeutic response of musculoskeletal disorders. Understanding roles of ferroptosis for controlling musculoskeletal diseases might provide directions for ferroptosis-driven therapies, which could be promising for the development of novel therapeutic strategies.
摘要:
In the ABL wind tunnel, three inverted umbrella-shaped membrane structure groups were subjected to simultaneous pressure measurement model test. Time series of the fluctuating wind pressure were collected from 24 different wind angles. Upon examining the correlation coefficient of the fluctuating wind pressure between the upper and lower surfaces at the same pressure tap, it was discovered that the fluctuating wind pressure of the upwind roof exhibited asynchronous changes, which is a characteristic of a negative correlation area and has detrimental effects on the structure's wind resistance. The local areas with non-Gaussian properties were evaluated by analyzing the probability density function of representative pressure taps. The mechanism behind non-Gaussian wind pressure was elucidated through considerations of the central limit theorem and spatial correlation of fluctuating wind pressure. This study introduces a quantitative classification criterion for identifying the non-Gaussian distribution area of fluctuating wind pressure, setting the threshold at an 80% cumulative probability for both skewness and kurtosis. The analysis showed that the steady airflow of the structure corresponds to the Gaussian distribution area, while the non-Gaussian distribution area is primarily concentrated at the front edge of the upwind roof and the rear edge of the downwind roof. To ascertain the peak factor for the non-Gaussian distribution, the improved peak factor method was utilized, providing insights into the range of peak factor in the non-Gaussian distribution area of this particular roof configuration. This information serves as a foundation for the wind resistance design of this structure.
作者:
Xi Zhong;Yong Peng;Ximei Zhang;Luogen Peng;Kaiwen Ma;...
期刊:
PATHOLOGY RESEARCH AND PRACTICE,2023年:154950 ISSN:0344-0338
通讯作者:
Xianghui Yang
作者机构:
[Xi Zhong; Yong Peng; Ximei Zhang; Kaiwen Ma; Yong Huang] Department of Oncology, People’s Hospital of Ningxiang, Changsha City, Hunan Province, China;[Luogen Peng; Xianghui Yang] Department of Oncology, The Affiliated Changsha Central Hospital,Hengyang Medical School, University of South China, Changsha City, Hunan Province, China
通讯机构:
[Xianghui Yang] D;Department of Oncology, The Affiliated Changsha Central Hospital,Hengyang Medical School, University of South China, Changsha City, Hunan Province, China
摘要:
Circular RNAs (circRNAs) are believed to regulate the progression of various cancers including colorectal cancer (CRC). However, the role and mechanism of circ_0124554 in regulating the sensitivity of CRC to radiation remain unknown.
The RNA levels of circ_0124554, LIM and SH3 protein 1 (LASP1), and methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit (METTL3) were detected by quantitative real-time polymerase chain reaction. Protein expression was checked by western blot. Cell proliferation, apoptosis, migration, and invasion were investigated by 5-Ethynyl-2’-deoxyuridine (EdU) assay, flow cytometry analysis, and transwell assay, respectively. The sensitivity of CRC cells to radiation was analyzed by cell colony formation assay. Xenograft mouse model assay was conducted to disclose the role of circ_0001023 in the sensitivity of tumors to radiation in vivo. The binding relationships among circ_0124554, miR-1184 and LASP1 were confirmed by a dual-luciferase reporter assay. m6A RNA immunoprecipitation assay was performed to identify the association of METTL3 with circ_0124554.
Circ_0124554 expression was upregulated in CRC tissues and cells in comparison with normal colorectal tissues and cells. Circ_0124554 knockdown inhibited proliferation, migration and invasion and promoted apoptosis and radiosensitivity of CRC cells. Moreover, circ_0124554 depletion inhibited tumor formation and improved radiosensitivity in vivo. MiR-1184 was identified as a target miRNA of circ_0124554 and targeted LASP1. Additionally, LASP1 overexpression rescued circ_0124554 knockdown-mediated effects in CRC cells. METTL3 mediated m6A methylation of circ_0124554. Further, circ_0124554 overexpression attenuated METTL3 depletion-induced effects in CRC cells.
m6A-modified circ_0124554 promoted CRC progression and radioresistance by inducing LASP1 expression through interaction with miR-1184.
作者机构:
[Xu, Ye; Liu, Rushi; Li, Xiaofeng; Zhong, Zhihong; Tan, Chufan; Li, Sang] Hunan Normal Univ, Sch Med, Lab Med Mol & Immunol Diagnost, Changsha 410013, Peoples R China.;[Qiu, Yilan; Qiu, YL] Hunan Normal Univ, Sch Life Sci, Changsha 410081, Peoples R China.;[Wu, Junwen] Nanya Middle Sch Changsha, Changsha 410129, Peoples R China.;[Feng, Bo; Feng, B] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Changsha 410008, Peoples R China.
通讯机构:
[Feng, B ] U;[Qiu, YL ] H;Hunan Normal Univ, Sch Life Sci, Changsha 410081, Peoples R China.;Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Changsha 410008, Peoples R China.
关键词:
Hepatitis E;HEV vaccine;Virus -like particles;The recombinant capsid protein p27
摘要:
HEV (Hepatitis E virus) is an infectious disease transmitted between humans and animals, which poses a severe threat to the biological safety and property throughout the world. The disease is especially severe in patients with potential liver cirrhosis and women during pregnancy. There is no specific and thorough HEV treatment at present. The development of hepatitis E virus vaccine is vital to the prevention of viral hepatitis worldwide. Since HEV cannot grow adequately in vitro, vaccine developed by devitalized virus particles does not work. Exploration of HEV-like structures is essential for the development of functional vaccines against HEV infection. ORF2 encodes the structural proteins of HEV, some of which can automatically assemble into virus-like particles (VLP) in this experiment, the recombinant capsid protein p27 was expressed in E. coli and the VLP formed by p27 was used to immunize mice. The results showed that the VLP formed by recom-binant P27 had similar particle size to that of HEV; the immune dose produced by p27 was positively correlated with the immune effect. Compared with other genetic engineering subunit vaccines, P27 protein has a better application prospect.
作者机构:
[金良; 陈勇军; 陈语婕] Department of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang 421002, China
关键词:
基因变异;发育性和癫痫性脑病;OMIM数据库;精准治疗
摘要:
发育性和癫痫性脑病(developmental and epileptic encephalopathy,DEE)是一组临床和遗传异质的年龄依赖性神经系统疾病,其特征是在婴儿期或儿童早期出现难治性癫痫发作,且受影响的个体...展开更多 发育性和癫痫性脑病(developmental and epileptic encephalopathy,DEE)是一组临床和遗传异质的年龄依赖性神经系统疾病,其特征是在婴儿期或儿童早期出现难治性癫痫发作,且受影响的个体有精神运动发育迟缓或倒退。随着二代测序技术的发展,尤其是全外显子测序技术的应用,越来越多的基因被发现与DEE相关。这些发现将为临床工作中DEE致病基因的检测提供依据,同时将有助于加深对DEE发病机制的理解。本文主要对DEE的遗传学病因及诊疗的相关研究进展展开综述,以期帮助临床医生早期识别相关基因突变,从而加快疾病诊断并及时实施最佳治疗。收起
摘要:
Heavy metal pollution seriously threatens the environment and human health. The biosorption of heavy metals has attracted worldwide attention due to its cost-effectiveness and environmental friendliness. It is significant to develop biosorbents with excellent adsorption performance. Sphingopyxis is widely used in the removal of various organic pollutants, but its potential application in heavy metal adsorption has been largely overlooked. This study investigates the biosorption of U(VI) onto live and dead cells of a Sphingopyxis strain YF1. The effects of pH, contact time and initial ion concentration on U(VI) adsorption investigated, and kinetic and isothermal models were used to fit the adsorption results. The results show that under pH 3-6, the adsorption of U(VI) by YF1 live cells increased with the increase of the pH. Both the pseudo-first order and pseudo-second order models can satisfactorily interpret the adsorption by live and dead cells. Three isothermal adsorption models (Langmuir, Freundlich, and Sips) were used to fit the adsorption process. The adsorption of uranium by live and dead cells was best fitted by the Sips model. The maximal adsorption capacities of U(VI) by live and dead cells were 140.7 mg g(-1) and 205.7 mg g(-1), respectively. The mechanisms of U(VI) adsorption by Sphingopyxis sp. YF1 were revealed. Scanning electron microscopy and energy dispersive spectroscopy (SEM-EDS) show that U(VI) was deposited on the surface of the bacterial cells. Fourier-transform infrared spectroscopy (FTIR) shows that amine, hydroxyl, alkyl, amide I, amide II, phosphate, carboxylates and carboxylic acids were the major functional groups that are involved in U(VI) adsorption by live and dead cells. X-ray photoelectron spectroscopy (XPS) suggests that the main functional groups of live cells involved in adsorption were O = C-O, C-OH/C-O-C and N-C = O. This study indicates Sphingopyxis sp. YF1 is a high-efficiency U(VI)-adsorbing strain, promising to remove U(VI) from aquatic environment.
作者机构:
[Li, Liuqin; Fang, Qi] School of Resource & Environment and Safety Engineering, University of South China, Hengyang, Hunan, 421001, PR China;[Zhu, Nengwu] School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou, Guangdong, 510006, PR China;Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, 421001, PR China;[Huang, Xixian] School of Resource & Environment and Safety Engineering, University of South China, Hengyang, Hunan, 421001, PR China<&wdkj&>School of Environment and Energy, Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, South China University of Technology, Guangzhou, Guangdong, 510006, PR China;[Ding, Yang] School of Resource & Environment and Safety Engineering, University of South China, Hengyang, Hunan, 421001, PR China<&wdkj&>Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, 421001, PR China
通讯机构:
[Yang Ding] S;School of Resource & Environment and Safety Engineering, University of South China, Hengyang, Hunan, 421001, PR China<&wdkj&>Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, 421001, PR China
摘要:
The dynamic reactions of uranium (U) with iron (Fe) minerals change its behaviors in soil environment, however, how the coexisted constituents in soil affect U sequestration and release on Fe minerals during the transformation remains unclear. Herein, coupled effects of lead (Pb) and dissolved organic matter (DOM) on U speciation and release kinetics during the catalytic transformations of ferrihydrite (Fh) by Fe(II) were investigated. Our results revealed that the coexistence of Pb and DOM significantly reduced U release and increased the immobilization of U during Fh transformation, which were attributed to the enhanced inhibition of Fh transformation, the declined release of DOM and the increased U(VI) reduction. Specifically, the presence of Pb increased the coprecipitation of condensed aromatics, polyphenols and phenols, and these molecules were preferentially maintained by Fe (oxyhydr)oxides. The sequestrated polyphenols and phenols could further facilitate U(VI) reduction to U(IV). Additionally, a higher Pb content in coprecipitates caused a slower U release, especially when DOM was present. Compared with Pb, the concentrations of the released U were significantly lower during the transformation. Our results contribute to predicting U sequestration and remediating U-contaminated soils.
摘要:
Investigating the tectonostratigraphic evolution of Late Mesozoic basins in NE China and its surrounding areas offers great opportunities to restore the Late Mesozoic evolution of intraplate tectonic stress regime in East Asia and to uncover the relationships between intraplate deformation and plate margin tectonic processes. This study provides a comprehensive analysis of the stratigraphic and structural evolution of the basins in NE China and its surrounding areas during the Late Mesozoic. It is shown that these basins underwent significant extension and syn-rift subsidence during the Late Jurassic-Early Cretaceous. However, the long-lived extension was intermittent rather than continuous, and at least three pulses of short-lived episodic compressive deformation, that occurred at the latest Jurassic-earliest Cretaceous, middle Aptian (ca. 120 Ma), and late Aptian (ca. 115 Ma), could be recognized. The Late Jurassic-Early Cretaceous extension was terminated by a strong contractional event at the earliest Late Cretaceous. The frequent switches in regional tectonic stress regime between extension and contraction require a new tectonic interpretation, as proposed here. We suggest that during the Late Jurassic-Early Cretaceous, the continental collision and subsequent northeastward indentation of the Karakoram-Lhasa Block with the southern Asian margin probably activated a significant strike-slip fault system, propagating approximately from the collision zone to the eastern Asian margin, and drove East Asia into a lateral extrusion/escape tectonic setting. The activated strike-slip faulting probably triggered the postorogenic gravitational collapse of the thickened upper crust and the delamination of the lower crust and mantle lithosphere, resulting in regional extension and intense magmatism in East Asia during the Late Mesozoic. The intercurrent multiphase short-lived contractional events were likely associated with a series of continental collision events along Asian margins. The latest Jurassic-earliest Cretaceous contractional deformation probably resulted from the combined effects of the closure of the eastern Mongol-Okhotsk Ocean, the Kolyma-Omolon/Siberia collision, and the Karakoram-Lhasa/Southern Asia collision. The middle Aptian contractional deformation probably resulted from the Proto-Kurosegawa/Eastern Asia collision. The late Aptian contractional deformation probably resulted from the Chukotka/Kolyma-Omolon collision. The earliest Late Cretaceous contractional deformation probably resulted from the Okhotomorsk/Eastern Asia collision.
作者机构:
[Yang, Xia; Yang, Xue-Feng] Hunan Provincial Clinical Research Center for Metabolic Associated Fatty Liver Disease;Affiliated Nanhua Hospital, Hengyang Medical School & University of South China, Hengyang, Hunan 421002, China;[Wang, Yuan-Yuan; Lin, Hai-Lian; Wang, Ke-Lang; Que, Gen-Xiang; Cao, Ting] Department of Gastroenterology;[Zhu, La-Mei] Department of Hepatobiliary Surgery;Department of General Practice
摘要:
Metabolic-associated fatty liver disease (MAFLD) has become a prevalent chronic liver disease worldwide because of lifestyle and dietary changes. The gut microbiota and its metabolites have been shown to play a critical role in the pathogenesis of MAFLD. Understanding of the function of the gut microbiota and its metabolites in MAFLD may help to elucidate pathological mechanisms, identify diagnostic markers, and develop drugs or probiotics for the treatment of MAFLD. Here we review the pathogenesis of MAFLD by gut microbiota and its metabolites and discuss the feasibility of treating MAFLD from the perspective of gut microbes.
摘要:
Contrast-enhanced ultrasound (CEUS) is a dependable modality for the diagnosis of various clinical conditions. A judicious selection of ultrasound contrast agent (UCA) is imperative for optimizing imaging and improving diagnosis. Approved UCAs for imaging the majority of organs include SonoVue, a pure blood agent, and Sonazoid, which exhibits an additional Kupffer phase. Despite the fact that the two UCAs are increasingly being employed, there is a lack of comparative reviews between the two agents in different organs diseases. This review represents the first attempt to compare the two UCAs in non-hepatic organs, primarily including breast, thyroid, pancreas, and spleen diseases. Through comparative analysis, this review provides a comprehensive and objective evaluation of the performance characteristics of SonoVue and Sonazoid, with the aim of offering valuable guidance for the clinical application of CEUS. Overall, further clinical evidences are required to compare and contrast the dissimilarities between the two UCAs in non-hepatic organs, enabling clinicians to make an appropriate selection based on actual clinical applications.
摘要:
It is a well-known phenomenon that natural products can serve as powerful drug leads to generate new molecular entities with novel therapeutic utility. Evodiamine (Evo), a major alkaloid component in traditional Chinese medicine Evodiae Fructus, is considered a desirable lead scaffold as its multifunctional pharmacological properties. Although natural Evo has suboptimal biological activity, poor pharmacokinetics, low water solubility, and chemical instability, medicinal chemists have succeeded in producing synthetic analogs that overshadow the deficiency of Evo in terms of further clinical application. Recently, several reviews on the synthesis, structural modification, mechanism pharmacological actions, structure-activity relationship (SAR) of Evo have been published, while few reviews that incorporates intensive structural basis and extensive SAR are reported. The purpose of this article is to review the structural basis, anti-cancer activities, and mechanisms of Evo and its derivatives. Emphasis will be placed on the optimizing strategies to improve the anticancer activities, such as structural modifications, pharmacophore combination and drug delivery systems. The current review would benefit further structural modifications of Evo to discover novel anticancer drugs.
通讯机构:
[Liu, ZG; Li, C ; Lin, YH ] X;Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res, Xiamen 361005, Peoples R China.;Xiamen Univ, Eye Inst, Sch Med, Fujian Prov Key Lab Ophthalmol & Visual Sci & Ocul, Xiamen 361102, Peoples R China.;Xiamen Univ, Affiliated Xiamen Eye Ctr, Sch Med, Xiamen 361102, Peoples R China.;Xiamen Univ, Natl Inst Data Sci Hlth & Med, Xiamen 361102, Peoples R China.
关键词:
Chemical ocular burn;Corneal diseases;Halogen;Metal–organic frameworks nanozyme;Superoxide dismutase
摘要:
Metal-organic frameworks (MOFs) have attracted significant research interest in biomimetic catalysis. However, the modulation of the activity of MOFs by precisely tuning the coordination of metal nodes is still a significant challenge. Inspired by metalloenzymes with well-defined coordination structures, a series of MOFs containing halogen-coordinated copper nodes (Cu-X MOFs, X = Cl, Br, I) are employed to elucidate their structure-activity relationship. Intriguingly, experimental and theoretical results strongly support that precisely tuning the coordination of halogen atoms directly regulates the enzyme-like activities of Cu-X MOFs by influencing the spatial configuration and electronic structure of the Cu active center. The optimal Cu-Cl MOF exhibits excellent superoxide dismutase-like activity with a specific activity one order of magnitude higher than the reported Cu-based nanozymes. More importantly, by performing enzyme-mimicking catalysis, the Cu-Cl MOF nanozyme can significantly scavenge reactive oxygen species and alleviate oxidative stress, thus effectively relieving ocular chemical burns. Mechanistically, the antioxidant and antiapoptotic properties of Cu-Cl MOF are achieved by regulating the NRF2 and JNK or P38 MAPK pathways. Our work provides a novel way to refine MOF nanozymes by directly engineering the coordination microenvironment and, more significantly, demonstrating their potential therapeutic effect in ophthalmic disease.