期刊:
Angewandte Chemie - International Edition,2024年 ISSN:1433-7851
摘要:
The aqueous electroreduction of nitric oxide (NO) to NH3 (NORR) has gained extensive attention for the sake of low carbon emission and air pollutant treatment. Unfortunately, NORR is greatly hindered by its sluggish kinetics on the electrode, especially under low concentrations of NO. Herein, we developed a chlorine (Cl) vacancy strategy to overcome this limitation over FeOCl nanosheets (FeOCl‐VCl). Density functional theory (DFT) calculations revealed that the Cl vacancy resulted in defective Fe with sharp d‐states characteristics in FeOCl‐VCl to enhance the absorption and activation of NO, enabling the conversion of NO to NH3 at low concentration. In situ XANES and ATR‐IR were carried out to verify the lower average oxidation state of defective Fe to enhance the electron transfer for NO adsorption/activation and facilitate the generation of key NHO and NHx intermediates. As a result, the FeOCl‐VCl exhibited superior NORR activities with the NH3 Faradaic efficiency up to 91.1% while maintaining a high NH3 yield rate of 455.4 μg cm‐2 h‐1 under 1.0 vol% low NO concentration, very competitive with those of previously reported catalytic systems under higher NO concentration. Further, the assembled Zn‐NO battery utilizing FeOCl‐VCl as cathode delivered a record peak power density of 6.2 mW cm‐2.
期刊:
Journal of Hazardous Materials,2024年465:133147 ISSN:0304-3894
通讯作者:
Chen, Lingxin;Xu, Jiakun
作者机构:
[Zhang, Weikang] State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao 266071, China;[Zhang, Weikang] College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;[Lin, Yingwu] School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;[Meng, Xiangmin] College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;[Wang, Qiaoning] CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
通讯机构:
[Chen, Lingxin] C;[Xu, Jiakun] S;CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China. Electronic address:;State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao 266071, China. Electronic address:
摘要:
Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., k(cat)/K(m) = 720.42M(-1) s(-1)) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45±0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation (k(cat)/K(m) = 670.08M(-1) s(-1)), while H64D/V68I had the best degradation rate against ST (i.e., 99.45±0.23%). Our results demonstrate that SAs can be efficiently degraded by artificial peroxygenases constructed using a myoglobin scaffold. This therefore provides a simple and economical method for the biodegradation of SD and ST.
作者机构:
[Wu, Jia; Li, Fusheng; Fang, Likui; Duan, Chongwen; Xu, Xiangyun; Wang, Dan; Chen, Shan] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.;[Tang, Weiyang] Hangzhou Normal Univ, Sch Pharm, Hangzhou 311121, Peoples R China.
通讯机构:
[Li, FS ] U;Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
摘要:
1,3-Enynes have been widely recognized as prevalent synthons for the construction of valuable allenes, 1,3-dienes, and propargylic derivatives. However, significant advancements have primarily focused on elucidating the ionic reaction mechanism. The selective difunctionalization of 1,3-enynes involving radicals remains an outstanding challenge in particular within the realm of radical 1,2-difunctionalization. Herein, we present a comprehensive account of the regio- and diastereoselective radical multicomponent 1,2-dialkylation of 1,3-enynes via photoexcited Hantzsch ester and chromium co-catalysis to furnish highly valuable homopropargylic alcohols. Our protocol shows excellent compatibility and 1,2-regioselectivity. Remarkably, this innovative approach obviates the need for external photocatalysts, stoichiometric metal reductants, or additional additives, offering a novel strategy for the valuable transformation of 1,3-enynes. We present a comprehensive account of the regio- and diastereoselective radical multicomponent 1,2-dialkylation of 1,3-enynes via photoexcited Hantzsch ester and chromium co-catalysis to furnish highly valuable homopropargylic alcohols.
通讯机构:
[Zhang, YZ ] S;Southern Univ Sci & Technol, Dept Chem, Shenzhen 518055, Peoples R China.
摘要:
Spin-crossover (SCO) complexes featuring hidden spin states have recently garnered significant attention. The hidden states are challenging to access via a routine cooling strategy due to the extremely slow dynamics of the SCO process, however, they are accessible via light irradiation. In this study, we prepared two homochiral mononuclear Fe-II complexes [(S(R)-L)Fe(NCBH3)(2)]center dot MeOH (S1 and R1, S/R-L = (S/R)-N-1,N-2-dibenzyl-N-1,N-2-bis(pyridin-2-ylmethyl)propane-1,2-diamine), as well as their desolvated counterparts, S1d and R1d, which can be interconverted through a single-crystal to single-crystal (SCSC) transformation (i.e., S1 <leftwards arrow with vertical stroke> S1d). All the complexes crystalize in the enantiomorphic polar space group of P2(1). The solvated complexes exhibit a thermally-induced spin crossover behavior at around 98 K and exhibit a common light-induced excited spin state trapping (LIESST) effect with a T-LIESST of 40 K. Meanwhile, the desolvated phases remain in the high-spin (HS) state above 2 K and show a rare reverse-LIESST effect, revealing a hidden low-spin (LS*) state. In addition, both the solvated and desolvated complexes exhibit bidirectional switching upon irradiation at 532 and 808 nm: one for LS <leftwards arrow with vertical stroke> HS* and the other for HS <leftwards arrow with vertical stroke> LS*. This work reveals a new finding regarding the influence of lattice solvents on light-induced magnetic bistability, providing a feasible approach for manipulating both thermally and photo-induced SCO events.
作者:
Ye, Yongjun*;Chen, Mengge;Chen, Guangling;Xia, Ming;Yu, Ting;...
期刊:
Journal of Environmental Radioactivity,2024年272:107353 ISSN:0265-931X
通讯作者:
Ye, Yongjun
作者机构:
[Ye, Yongjun; Xia, Ming; Wang, Haofeng; Chen, Mengge; Chen, Guangling; Yu, Ting] National Joint Engineering Research Center for Airborne Pollutants Control and Radiological Protection in Building Environment University of South China, Hengyang, 421001, Hunan, China;[Ye, Yongjun] Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 421001, Hunan, China. Electronic address: yongjunye@163.com
通讯机构:
[Ye, Yongjun] L;Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 421001, Hunan, China. Electronic address:
摘要:
There is a significant impact of the radon diffusion coefficient and the free radon production rate on the exhalation of radon from porous materials that can be regarded as spheres, hexahedrons, or cylinders. To understand this effect, the radon exhalation rules of spherical porous media with different radii were studied according to the radon diffusion migration theory. A specialized method for simultaneous determination of the radon diffusion coefficient and the free radon production rate of the spherical porous media was proposed, and applied to determine the above two parameters for two hemispherical test blocks with different radii. The results show that:(1) For spherical porous media with a certain radon diffusion length (L(d)), as the radius (r(0)) of the sphere increases, the radon exhalation rate first increases, and tends to stabilize at r(0)≥6L(d); The free radon release share gradually decreases from approximately 1, and drops to a steady state at r(0)≥18L(d). (2) Compared with conventional methods, the relative error of the free radon production rate determined by the proposed method is within 3.9%, which verifies the reliability of the new method.
期刊:
Journal of Hazardous Materials,2024年464:132986 ISSN:0304-3894
通讯作者:
Wang, Jikai
作者机构:
[Zhu, Yanli] School of Resources and Environment, Hunan University of Technology and Business, Changsha 410205, Hunan, PR China;[Wang, Jikai] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China. Electronic address: jkwang@hnu.edu.cn;[Xie, Zhulan; Sun, Yiyang; Zeng, Pengfei] Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China;[Chen, Danjun; Fu, Chengxiao] The First Affiliated Hospital, Department of Pharmacy, Hengyang Clinical Pharmacology Research Center, Hengyang Medical School, University of South China, Hengyang, 421001 Hunan, PR China;[Jiang, Yuehua] Department for Animal Husbandry & Aquaculture Products Quality Control, Hengyang Animal Husbandry and Aquaculture Affairs Center, Hengyang 421001, Hunan, PR China
通讯机构:
[Wang, Jikai] H;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Institute of Pharmacy & Pharmacology, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, PR China. Electronic address:
关键词:
Desorption ionization mechanism;Environmental analysis;MALDI-MS;Nano-matrix;Two-dimensional material
摘要:
Laser desorption ionization mass spectrometry (LDI-MS) aroused intensive concerns for the merits of label-free and high-throughput analysis. Here, we designed a silver nanoparticles (AgNP)-modified indium vanadate nanosheets with doping samarium (AgNP@InVO(4):Sm) nanosheets. The developed AgNP@InVO(4):Sm nanosheets (AIVON) were synthesized based on the microemulsion-mediated solvothermal method and ultraviolet-assisted in situ formation of AgNP, then for the first time applied as a matrix in LDI-MS analysis. With the advantages including enhanced MS signal, little matrix-related background, high reproducibility, and good salt tolerance, AIVON exhibited much better prospect than non-modified indium vanadate nanosheets with doping samarium (IVON) and traditional organic matrix, thus allowing sensitive MS detection for a wide range of low-molecular-weight (LMW) molecules. Moreover, by coupling with headspace sampling thin-film microextraction (TFME), a kind of representative pollutant chlorophenols were identified and quantified via AIVON-assisted LDI-MS in environmental and biological samples. Volatile LMW pollutants could be preconcentrated after TFME, hence a sensitive and rapid assay with negligible sample matrix effect was realized by using AIVON-assisted LDI-MS. It is anticipated that this novel nano-matrix AIVON and the proposed TFME coupling detection strategy were of competitive merits for LDI-MS analysis in the fields of environment, biomedicine, and agriculture.
作者机构:
[Sun, Xing; Liu, Dandan; Wen, Chengcai] Department of Hematology, the Second Xiangya Hospital, Molecular Biology Research Center, Center for Medical Genetics, School of Life Sciences, Hunan Province Key Laboratory of Basic and Applied Hematology, Central South University, Changsha, Hunan 410011, China;[Chen, Lu] Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;[Ye, Mao] Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China;[Liu, Hong] Department of Dermatology, Hunan Key Laboratory of Skin Cancer and Psoriasis, Hunan Engineering Research Center of Skin Health and Disease, Xiangya Clinical Research Center for Cancer Immunotherapy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;[Liang, Long] Department of Hematology, the Second Xiangya Hospital, Molecular Biology Research Center, Center for Medical Genetics, School of Life Sciences, Hunan Province Key Laboratory of Basic and Applied Hematology, Central South University, Changsha, Hunan 410011, China. Electronic address: liang_long614@126.com
通讯机构:
[Chang, Shi; Liu, Jing; Liang, Long] D;[Zhang, Ji] T;Department of Hematology, the Second Xiangya Hospital, Molecular Biology Research Center, Center for Medical Genetics, School of Life Sciences, Hunan Province Key Laboratory of Basic and Applied Hematology, Central South University, Changsha, Hunan 410011, China. Electronic address:;The Affiliated Nanhua Hospital, Department of Clinical Laboratory, Hengyang Medical School, University of South China, Hengyang, Hunan 421002, China. Electronic address:;Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China. Electronic address:
摘要:
Targeting immune checkpoint PD-1 or its ligand PD-L1 blockade has achieved a great therapeutic effect in a variety of cancer types. However, the overall response rate and duration are still limited for intrinsic and acquired resistance. There is an urgent need to understand the underlying mechanism. Studies showed that PD-L1 regulation is related to the response to PD-1 monoclonal antibodies (PD-1 mAB). Interestingly, emerging studies found that the different distribution of PD-L1 has distinct functions in tumor through the specific signaling pathways. Thus, controlling the distribution of PD-L1 provides an attractive therapeutic strategy for enhancing PD-1 mAB efficiency and rewiring the resistance. Here, we review the recent studies about the role and regulation of PD-L1 distribution from synthesis to surface delivery, internalization, recycling, or lysosome degradation and translocated into the nucleus or secreted into the extracellular space. We place this knowledge in the context of observations in the clinic and discuss the potential therapeutic strategies to enhance the efficacy of anti-PD-1/PD-L1 therapy.
作者机构:
[Liu, Hongliang] School of Mathematics and Physics, University of South China, Hengyang, 421001, PR China;[Cheng, Jun] School of Mathematics and Statistics, Guangxi Normal University, Guilin, 541004, PR China. Electronic address: jcheng@gxnu.edu.cn;[Cao, Jinde] School of Mathematics, Southeast University, Nanjing 210096, PR China;[Katib, Iyad] Department of Computer Science, Faculty of Computing and Information Technology, King Abdulaziz University, Jeddah 21589, Saudi Arabia
通讯机构:
[Cheng, Jun] S;School of Mathematics and Statistics, Guangxi Normal University, Guilin, 541004, PR China. Electronic address:
摘要:
This study addresses the preassigned-time synchronization for complex-valued memristive neural networks with reaction-diffusion terms and Markov parameters. Employing a preassigned-time stable control strategy, two distinct controllers with varying power exponent parameters are designed to ensure that synchronization can be achieved within a predefined time frame. Unlike existing finite/fixed-time results, a priori specification of the settling time is addressed. Furthermore, Green's formula and boundary conditions are efficiently applied to overcome potential symmetry loss. Additionally, the activation function's constraint range is more lenient compared to existing constraints. Finally, the effectiveness of the presented methods are demonstrated through two examples.
作者机构:
[Wang, Cong; Kang, Jiawei; Wu, Yifan] Department of Orthopaedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, 310009, Zhejiang Province, People's Republic of China;[Qin, Yating; Li, Yaping] College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China;[Qin, Yating; Li, Yaping] ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, 311215, Zhejiang, People's Republic of China;[Huang, Zhongming] The Affiliated Nanhua Hospital, Orthopedic Research Centre, Hengyang Medical School, University of South China, Hengyang, 421001, Hunan, People's Republic of China;[Sun, Long] Department of Radiology, Jining No. 1 People's Hospital, Jining Medical University, Jining, 272000, Shandong, People's Republic of China
通讯机构:
[Wang, Wei] C;[Feng, Gang; Qi, Yiying] D;College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, People's Republic of China.;ZJU-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, 311215, Zhejiang, People's Republic of China.;Department of Orthopaedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou City, 310009, Zhejiang Province, People's Republic of China.
摘要:
The integrated repair of bone and cartilage boasts advantages for osteochondral restoration such as a long-term repair effect and less deterioration compared to repairing cartilage alone. Constructing multifactorial, spatially oriented scaffolds to stimulate osteochondral regeneration, has immense significance. Herein, targeted drugs, namely kartogenin@polydopamine (KGN@PDA) nanoparticles for cartilage repair and miRNA@calcium phosphate (miRNA@CaP) NPs for bone regeneration, were in situ deposited on a patterned supramolecular-assembled 2-ureido-4 [lH]-pyrimidinone (UPy) modified gelation hydrogel film, facilitated by the dynamic and responsive coordination and complexation of metal ions and their ligands. This hydrogel film can be rolled into a cylindrical plug, mimicking the Haversian canal structure of natural bone. The resultant hydrogel demonstrates stable mechanical properties, a self-healing ability, a high capability for reactive oxygen species capture, and controlled release of KGN and miR-26a. In vitro, KGN@PDA and miRNA@CaP promote chondrogenic and osteogenic differentiation of mesenchymal stem cells via the JNK/RUNX1 and GSK-3β/β-catenin pathways, respectively. In vivo, the osteochondral plug exhibits optimal subchondral bone and cartilage regeneration, evidenced by a significant increase in glycosaminoglycan and collagen accumulation in specific zones, along withthe successful integration of neocartilage with subchondral bone. This biomaterial delivery approach represents a significant toward improved osteochondral repair.
作者机构:
[Zhang, Jing; He, Rongfang; Li, Jianping; Li, JP; Lan, Zhihua] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pathol, Hengyang, Peoples R China.
通讯机构:
[Li, JP ] U;Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Dept Pathol, Hengyang, Peoples R China.
关键词:
axillary lymph node;breast cancer patients;fine-needle aspiration;GATA-3 immunocytochemistry
摘要:
Background: Ultrasound-guided fine-needle aspiration cytology (FNAC) is a routine preoperative method for evaluating suspicious axillary lymph nodes (ALNs) in patients with breast cancer. However, a range of reasons such as morphological pitfalls, technical artifacts, and sampling errors restrict the sensitivity and accuracy of FNAC. This retrospective study investigated the diagnostic value of GATA-binding protein 3 (GATA-3) immunocytochemistry for FNAC. Methods: Breast cancer patients who underwent preoperative FNAC for suspicious ALNs, relevant GATA-3 immunocytochemistry, and postoperative status of ALNs were reviewed from the period of March 2020 to February 2022. Altogether, 102 patients were included in the study. FNAC material smears stained with hematoxylin and eosin was initially assessed by two cytopathologists and categorized into five groups: nondiagnostic, negative, atypical, suspicious, and positive for malignancy. Only group of cells positive for malignancy was considered positive. For each case, two selected slides were digitized (whole slide imaged) at x40 magnification and decolored for GATA-3 immunocytochemistry. The expression of GATA-3 was scored ranging from 0 to 9 (Score >= 3: Positive, Score <= 2: Negative). If either FNAC or GATA-3 immunocytochemistry was positive or the combined test positive, then the case was considered positive. The sensitivity, specificity, and accuracy of FNAC, GATA-3 immunocytochemistry, and combined FNAC/GATA-3 immunocytochemistry were analyzed by chi 2 and Fisher's tests. Results: The mean age of the study participants was 50.62 (ranging: 30-73 years). Invasive breast carcinoma (not otherwise specified) accounted for most histological subtypes, and grade 2 was the leading Nottingham grade. Sixteen cases directly underwent mastectomy while the other 86 patients had neoadjuvant therapy. A more serious diagnosis was made based on GATA-3 detection in 22.5% (n = 23) of 102 cases. Of the 23 cases, metastasis was confirmed by GATA-3 detection in 21 cases, and an uncertain diagnosis was ascertained based on GATA-3 immunocytochemistry in 2 with nondiagnostic FNAC results. The sensitivity (77/87, 88.5%) of GATA-3 detection for distinguishing malignancies from benign lesions was higher than that of FNAC alone (62/87, 71.3%) (p < .05). Conclusions: GATA-3 immunocytochemistry exhibited high diagnostic efficacy in distinguishing malignant breast cancer cells. Moreover, combined FNAC and GATA-3 immunocytochemistry achieved optimal results in terms of reducing the false-negative rate and promoting accuracy.
作者:
Liu, Yan;Wang, Zhen;Tang, Zhi;Fu, Yao;Wang, Lei
期刊:
Current Cancer Drug Targets,2024年 ISSN:1568-0096
作者机构:
[Liu, Yan; Wang, Zhen] Department of Neurology, Changsha Central Hospital, University of South China. No.161 Shaoshan road, Yuhua district, Changsha 410007, Hunan, China;[Tang, Zhi; Wang, Lei] Department of Neurosurgery, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University. No. 283 Tongzipo Road, Yuelu District, Changsha 410006, Hunan, China;[Fu, Yao] Department of Neurosurgery, Yiyang Central Hospital. No.118 North Kangfu Road, Heshan District,Yiyang 413000, Hunan, China
关键词:
Akt/mTOR pathway;Glioma;VEGFA;miRNA383-5p
摘要:
BACKGROUND: Previously, we have screened 59 differentially expressed miRNAs and 419 mRNAs in the glioblastoma samples that have been compared to the peritumoral tissues using bioinformatics analyses, which included miRNA-383-5p and vascular endothelial growth factor A (VEGFA). miRNA-383-5p and VEGFA/Akt/mTOR pathway play important regulatory roles in the malignant biological behavior of glioma. METHODS: Glioma cell lines, U87 and U251, were collected for in vitro experiments. miRNA-383-5p and VEGFA expression levels were detected with qRT-PCR and WB. The protein expressions of Akt, mTOR, and VEGFR in U87 and U251 were detected with WB. The effect of miRNA-383-5p on the VEGFA activity was verified by dual-luciferase reporter assay. CCK-8 was used to examine the U87 and U251 cells' inhibition. Flow cytometry and transwell assays were used to detect cell apoptosis and invasion, respectively. RESULTS: Our research data indicated overexpression of miRNA-383-5p to suppress malignant biological behavior, which was manifested as promoting the apoptosis of U87 and U251 cells and inhibiting invasion, proliferation, and metastasis. VEGFA is one of the downstream target genes of miRNA-383-5p. miRNA-383-5p could inhibit the expression of VEGFA and Akt/mTOR signaling pathways. Overexpression of VEGFA can reverse the inhibitory effect of miRNA-383-5p and reactivate the Akt/mTOR signaling pathway. CONCLUSION: Our results indicate that miRNA-383-5p functions as an anti-oncogene by inhibiting the VEGFA/Akt/mTOR signaling pathway in glioma cells. These data provide potential therapeutic targets for glioblastoma.
期刊:
Frontiers in Cell and Developmental Biology,2024年11:1305433 ISSN:2296-634X
作者机构:
[Han, Lei; Shi, Dazhi; Wang, Zijing; Shu, Yilai] Department of Otorhinolaryngology, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, China;[Wang, Daqi; Hu, Shaowei; Han, Lei; Wang, Zijing; Gao, Ziwen; Shu, Yilai] ENT Institute and Department of Otorhinolaryngology, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China;[Wang, Daqi; Hu, Shaowei; Han, Lei; Wang, Zijing; Gao, Ziwen; Shu, Yilai] Institutes of Biomedical Sciences, Fudan University, Shanghai, China;[Wang, Daqi; Hu, Shaowei; Han, Lei; Wang, Zijing; Gao, Ziwen; Shu, Yilai] NHC Key Laboratory of Hearing Medicine, Fudan University, Shanghai, China
关键词:
aminoglycosides;gene therapy;hair cell;ototoxicity;programmed cell death
摘要:
Aminoglycosides are commonly used for the treatment of life-threatening bacterial infections, however, aminoglycosides may cause irreversible hearing loss with a long-term clinical therapy. The mechanism and prevention of the ototoxicity of aminoglycosides are still limited although amounts of studies explored widely. Specifically, advancements in programmed cell death (PCD) provide more new perspectives. This review summarizes the general signal pathways in programmed cell death, including apoptosis, autophagy, and ferroptosis, as well as the mechanisms of aminoglycoside-induced ototoxicity. Additionally, novel interventions, especially gene therapy strategies, are also investigated for the prevention or treatment of aminoglycoside-induced hearing loss with prospective clinical applications.
作者机构:
[Wei Wang] University of South China, Zhengxiang District, Hengyang City, Hunan Province, Hengyang, Hunan, 421001, CHINA;[Bo Zheng] School of Nuclear Science and Technology, University of South China, University of South China, Hengyang, 421001, People's Republic of China, Hengyang, 421001, CHINA;[Jiajia Qin] University of South China, Hengyang, Hengyang, Hunan, 421001, CHINA;[勇 至 王#Wei Wang] Institute of High Energy Physics Chinese Academy of Sciences, 北京918信箱实验物理中心, Beijing, 100049, CHINA
摘要:
The identification of deuteron with momentum in range of $0.52$-$0.72$ GeV/$c$ has been studied with the specific ionization energy loss information, using the data sample collected by the BESIII detector at center-of-mass energies between $4.009$ and $4.946$ GeV. Clean deuteron samples are selected using time of flight information. For all data samples, the deuteron identification efficiencies are higher than $95\%$, with a maximum difference of $(4.93\pm0.95)\%$ between data and MC simulation. These verify the effectiveness of the deuteron identification method based on the specific ionization energy loss, and provide valuable information for the future studies of the processes involving deuteron in the final state at BESIII. Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. Article funded by SCOAP3 and published under licence by Chinese Physical Society and the Institute of High Energy Physics of the Chinese Academy of Science and the Institute of Modern Physics of the Chinese Academy of Sciences and IOP Publishing Ltd.
期刊:
Measurement Science And Technology,2024年 ISSN:0957-0233
作者机构:
[Pingping Wang] University of South China, Yumu Campus, University of South China, No.228 Hengqi Road, Yumushan Town, Steaming Xiang District, Hengyang City, Hunan Province, China., 衡阳市, 421200, CHINA;[Jiahua Chen] University of South China, Yumu Campus, University of South China, No.228 Hengqi Road, Yumushan Town, Steaming Xiang District, Hengyang City, Hunan Province, China., Hengyang, 421001, CHINA
摘要:
Spent fuel shearing machines in nuclear power plants are important equipment for the head end of spent fuel reprocessing in power reactors. Condition monitoring and fault diagnosis play important roles in ensuring the safe operation of spent fuel shearing machines, avoiding serious accidents, and reducing their maintenance time and cost. Existing research on fault diagnosis of spent fuel shearing machiness has some shortcomings: (a) the current research on fault diagnosis of shearing machines is small and diagnostic accuray is not high. The research methodology of shearing machines needs to be updated; (b) the high difficulty in obtaining fault data and the often limited and highly informative fault data for shearing machines lead to low diagnostic performance. To solve these problems, this study constructs a residual network model based on Bayesian optimization and CBAM. First, dual-channel difference method is introduced into the preprocessing of noise signals, and two data enhancements were applied to the Mel spectrograms used as inputs to the model. Second, the attention mechanism CBAM is introduced to improve the residual network to enhance the deep feature extraction ability of the network, and the Bayesian optimization algorithm is used to train the hyperparameters, such as the optimizer, and retrain the network model after obtaining the optimal hyperparameters. Finally, the feasibility and effectiveness of the proposed model are verified through experiments on the noise signals of spent fuel shearing machines. The experimental results show that the diagnostic accuracy of the constructed model is 93.67%, which is a significant improvement over the other methods.
作者机构:
[Feng, Yong; Kang, Xiaoming; Gong, Wei; Jing, Qiancheng; Feng, Zhili; Wen, Jie] Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Otorhinolaryngol, Changsha, Peoples R China.;[Feng, Yong; Ma, Lu; Kang, Xiaoming; Gong, Wei; Jing, Qiancheng; Feng, Zhili; Wen, Jie] Univ South China, Inst Otorhinolaryngol Head & Neck Surg, Changsha, Peoples R China.;[Mei, Lingyun; Song, Jian; He, Chufeng] Cent South Univ, Dept Otorhinolaryngol, Xiangya Hosp, Changsha, Peoples R China.;[Feng, Yong; Mei, Lingyun; Song, Jian; He, Chufeng] Prov Key Lab Otolaryngol Crit Dis, Changsha, Peoples R China.;[Chen, Jiale] Cent South Univ, Sch Life Sci, Hunan Key Lab Med Genet, Changsha, Peoples R China.
通讯机构:
[Ma, L; Feng, Y ] ;Univ South China, Affiliated Changsha Cent Hosp, Hengyang Med Sch, Dept Otorhinolaryngol, Changsha, Peoples R China.;Univ South China, Inst Otorhinolaryngol Head & Neck Surg, Changsha, Peoples R China.
摘要:
Waardenburg Syndrome (WS) is a rare genetic disorder that leads to congenital hearing loss and pigmentation defects. Microphthalmia-associated transcription factor (MITF) is one of its significant pathogenic genes. Despite the comprehensive investigation in animal models, the pathogenic mechanism is still poorly described in humans due to difficulties accessing embryonic tissues. In this work, we used induced pluripotent stem cells derived from a WS patient carrying a heterozygous mutation in the MITF gene c.626A>T (p.His209Leu), and differentiated toward melanocyte lineage, which is the most affected cell type involved in WS. Compared with the wild-type cell line, the MITFmut cell line showed a reduced expression of the characteristic melanocyte-related genes and a lesser proportion of mature, fully pigmented melanosomes. The transcriptome analysis also revealed widespread gene expression changes at the melanocyte stage in the MITFmut cell line. The differentially expressed genes were enriched in melanogenesis and cell proliferation-related pathways. Interestingly, ion transport-related genes also showed a significant difference in MITFmut-induced melanocytes, indicating that the MITF mutant may lead to the dysfunction of potassium channels and transporters produced by intermediate cells in the cochlea, further causing the associated phenotype of deafness. Altogether, our study provides valuable insights into how MITF mutation affects WS patients, which might result in defective melanocyte development and the related phenotype based on the patient-derived iPSC model.