期刊:
Applied Mechanics and Materials,2012年204-208:3776-3781 ISSN:1660-9336
通讯作者:
Wu, Xianlei
作者机构:
[Shi, Jianjun; Shan, Zhi; Wu, Xianlei] Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
通讯机构:
[Wu, Xianlei] U;Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
会议名称:
International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2012)
会议时间:
AUG 10-12, 2012
会议地点:
Zhangjiajie, PEOPLES R CHINA
会议主办单位:
[Wu, Xianlei;Shi, Jianjun;Shan, Zhi] Univ South China, Sch Urban Construct, Hengyang 421001, Peoples R China.
会议论文集名称:
Applied Mechanics and Materials
关键词:
MATLAB image process technique;Rheological constants;SCC;V-Funnel
摘要:
This template is mainly about the analysis of mechanical property of SCC in a flow state in the V-Funnel. It aims to establish the testing formula of rheological constants by Engineering Fluid Mechanics, which can provide academic basis for V-Funnel test of SCC properties. MATLAB image process technique was introduced in the process which can greatly improve its precision and reliability. It can also provide a new train of thought to experimental data.
作者:
Ling, H. -y.;Hu, B.;Hu, X. -b.;Zhong, J.;Feng, S. -d.;...
期刊:
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES,2012年120(9):553-559 ISSN:0947-7349
通讯作者:
Liao, D. -f.
作者机构:
[Ling, H. -y.; Hu, B.] Univ S China, Sch Med, Dept Physiol, Hengyang, Peoples R China.;[Liao, D. -f.] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.;[Ling, H. -y.] Univ S China, Ctr Basic Med Postdoctoral Studies, Hengyang, Peoples R China.;[Hu, X. -b.] Univ S China, Sch Life Sci & Technol, Dept Biochem & Mol Biol, Hengyang, Peoples R China.;[Wen, G. -b.; Zhong, J.] Univ S China, Affiliated Hosp 1, Inst Clin Res, Hengyang, Peoples R China.
通讯机构:
[Liao, D. -f.] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.
摘要:
A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3'-end of FDNA. The fluorescence signal "turning on" was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20 mmol L-1 Tris-HCl buffer solution of pH 7.4, Delta F signal linearly correlated with the concentration of Ade over the range of 2.0 x 10(-8) to 1.8 x 10(-6) mol L-1. The limit of detection (LOD) for Ade is 6.0 x 10(-9) mol L-1 with relative standard deviations (R.S.D) of 3.64-5.36%, and the recoveries were 98.6%, 100%, 102% (n = 6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique. (C) 2012 Elsevier B.V. All rights reserved.
关键词:
coupled heat and moisture transfer;transient moisture content;multi-layer porous building envelope
摘要:
A new facility was presented which can expediently and cheaply measure the transient moisture content profile in multi-layer porous building envelope. Then, a common multi-layer porous building envelope was provided, which was constructed by cement mortar-red brick-cement plaster. With this kind of building envelope installed in the south wall, a well-controlled air-conditioning room was set up in Changsha, which is one of typical zones of hot and humid climate in China. And experiments were carried out to investigate the temperature and moisture distribution in multi-layer building envelope in summer, both in sunny day and rainy day. The results show that, the temperature and humidity at the interface between the brick and cement mortar are seriously affected by the changes of outdoor temperature and humidity, and the relative humidity at this interface keeps more than 80% for a long-term, which can easily trigger the growth of mould. The temperature and humidity at the interface between the brick and cement plaster change a little, and they are affected by the changes of indoor temperature and humidity. The temperature and humidity at the interface of the wall whose interior surface is affixed with a foam plastic wallpaper are generally higher than those of the wall without wallpaper. The heat transfer and moisture transfer in the envelope are coupled strongly.